Document 0424
 DOCN  M9490424
 TI    Human immunodeficiency virus type 1 Rev is required in vivo for binding
       of poly(A)-binding protein to Rev-dependent RNAs.
 DT    9411
 AU    Campbell LH; Borg KT; Haines JK; Moon RT; Schoenberg DR; Arrigo SJ;
       Department of Microbiology and Immunology, Medical University of; South
       Carolina, Charleston 29425-2230.
 SO    J Virol. 1994 Sep;68(9):5433-8. Unique Identifier : AIDSLINE
       MED/94335055
 AB    In the absence of Rev or the Rev-responsive element, the Rev-dependent
       human immunodeficiency virus type 1 (HIV-1) RNAs do not behave as mRNAs;
       rather, they exhibit nuclear defects in splicing and/or nuclear export
       and cytoplasmic defects in stability and translation. A translational
       initiation factor, eIF-5A, has recently been shown to bind specifically
       to the Rev activation domain. As the binding of poly(A)-binding protein
       1 (PAB1) to the poly(A) tail of mRNAs is involved in both the stability
       and translation of cytoplasmic mRNAs, we investigated whether Rev might
       influence the association of PAB1 with cytoplasmic HIV-1 RNAs.
       Antibodies were generated against PAB1. We used these antibodies in an
       immunoprecipitation assay to detect specific binding of PAB1 to
       cytoplasmic mRNAs. We found that in the presence of Rev, PAB1 was
       associated with Rev-dependent and Rev-independent RNAs in the cytoplasm
       of transfected cells. However, in the absence of functional Rev, we
       found little or no PAB1 associated with Rev-dependent RNAs. These RNAs
       were capable of binding PAB1 in vitro. These results demonstrate that
       HIV-1 RNAs are defective in PAB1 association in the absence of Rev.
 DE    Amino Acid Sequence  Base Sequence  Cell Line  Cytoplasm/METABOLISM
       *Gene Expression Regulation, Viral  Gene Products, rev/*METABOLISM
       Human  HIV-1/*GENETICS  In Vitro  Molecular Sequence Data
       Oligonucleotide Probes/CHEMISTRY  Peptides/CHEMISTRY  RNA-Binding
       Proteins/*METABOLISM  RNA, Messenger/METABOLISM  RNA, Viral/METABOLISM
       Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  Translation,
       Genetic  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).