Document 0450
 DOCN  M9490450
 TI    Analysis of the cytolytic activity mediated by natural killer cells from
       acquired immunodeficiency syndrome patients in response to
       phytohemagglutinin or anti-CD16 monoclonal antibody.
 DT    9411
 AU    Sirianni MC; Mezzaroma I; Aiuti F; Moretta A; Department of Allergy and
       Clinical Immunology, University of Rome; La Sapienza, Italy.
 SO    Eur J Immunol. 1994 Aug;24(8):1874-8. Unique Identifier : AIDSLINE
       MED/94333486
 AB    The aim of this study was to assess the cytolytic potential of natural
       killer (NK) cells from human immunodeficiency virus type 1
       (HIV-1)-infected patients, at different stages of the disease. Twenty
       HIV-1 seronegative donors as well as sixty HIV-1 seropositive patients
       were studied. Phytohemagglutinin and/or the anti-CD16 monoclonal
       antibody Kd1 were used to redirect the peripheral blood lymphocyte lysis
       of these patients to the 51Cr-labeled Fc gamma receptor-positive P815
       murine mastocytoma target cell line. In parallel, NK cytotoxicity to
       tumor targets was investigated. Seronegative as well as HIV-1 Center for
       Disease Control (CDC) stage II patients showed maintained cytolytic
       activity. The cytolytic potential declined with disease progression,
       starting with CDC IVC2 patients, and was strongly diminished in acquired
       immunodeficiency syndrome stage patients. This defect was accompanied by
       decreased cytolytic activity to tumor targets and was not corrected by
       the in vitro addition of interleukin-2. The number of cells bearing a
       mature NK phenotype was normal in all the study groups. Our data suggest
       that the impaired NK cytotoxicity to tumor targets described during the
       progression of HIV-1 disease may be related to the progressive loss of
       function of surface receptors involved in NK cell triggering.
 DE    Acquired Immunodeficiency Syndrome/*IMMUNOLOGY  Antibodies, Monoclonal
       Cells, Cultured  Cytotoxicity, Immunologic/*IMMUNOLOGY  Flow Cytometry
       Human  Immunophenotyping  Interleukin-2/IMMUNOLOGY  Killer Cells,
       Natural/*IMMUNOLOGY  Lymphocyte Transformation
       Phytohemagglutinins/PHARMACOLOGY  Receptors, IgG/*IMMUNOLOGY  Support,
       Non-U.S. Gov't  Tumor Cells, Cultured/IMMUNOLOGY  JOURNAL ARTICLE

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