Document 0476
 DOCN  M9490476
 TI    Preparative scale culture of Escherichia coli cells expressing the human
       immunodeficiency virus type 1 Tat protein.
 DT    9411
 AU    McKenna MC; Muchardt C; Gaynor R; Eisenberg D; Molecular Biology
       Institute, University of California at Los; Angeles 90024.
 SO    Protein Expr Purif. 1994 Apr;5(2):105-11. Unique Identifier : AIDSLINE
       MED/94331968
 AB    A procedure leading to a 100-liter fermentor culture of Escherichia coli
       cells expressing the human immunodeficiency virus type 1 (HIV-1)
       trans-activator (Tat) protein is described. The effects of growth
       temperature and of cell density at the time of induction on the yield of
       Tat were investigated. Tat was identified by SDS-gel electrophoresis and
       Western blot. Tat represents approximately 10% of the soluble protein in
       the cell lysate.
 DE    Amino Acid Sequence  Escherichia coli/*GROWTH &
       DEVELOPMENT/GENETICS/METABOLISM  Fermentation  Gene Products,
       tat/BIOSYNTHESIS/*GENETICS/ISOLATION & PURIF  Genes, tat  Genes,
       Synthetic  HIV-1/*GENETICS  Industrial Microbiology/*METHODS  Molecular
       Sequence Data  Recombinant Fusion
       Proteins/BIOSYNTHESIS/*GENETICS/ISOLATION &  PURIF  Support, U.S. Gov't,
       P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).