Document 0515
 DOCN  M9490515
 TI    The ratio of defective HIV-1 particles to replication-competent
       infectious virions.
 DT    9411
 AU    Bourinbaiar AS; Department of Biochemistry, NYU Medical Center, NY
       10016.
 SO    Acta Virol. 1994 Feb;38(1):59-61. Unique Identifier : AIDSLINE
       MED/94346346
 AB    The ratio of infectious to defective particles has been investigated for
       human immunodeficiency virus type 1 (HIV-1). Although the concentration
       of HIV core p24 protein defined by ELISA permits the estimation of a
       total number of average-sized HIV particles, it does not provide
       information on the numerical value of infectious particles. This problem
       was addressed by limiting dilution of viral supernatant derived from
       various infected cell lines and then comparing the total number of HIV
       particles to the end-point titers of viral inoculum determined by
       reverse transcriptase assay and virus-induced cytotoxicity. The results
       indicate that the ratio of infectious to defective virus particles
       varies for different virus strains and host cells within a range from
       1:1 to 1:100, but for given virus-cell system it remains constant.
 DE    Cell Line  Culture Media/CHEMISTRY  Cytopathogenic Effect, Viral
       Defective Viruses/*PHYSIOLOGY  Human  HIV Core Protein p24/ANALYSIS
       HIV-1/*PHYSIOLOGY  Monocytes/METABOLISM  Reverse Transcriptase/ANALYSIS
       Support, U.S. Gov't, P.H.S.  T-Lymphocytes/MICROBIOLOGY
       Virion/*PHYSIOLOGY  Virus Replication/*PHYSIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).