Document 0522
 DOCN  M9490522
 TI    [The use of modified polystyrene plates in solid-phase immunoenzyme
       analysis for the diagnosis of infections]
 DT    9411
 AU    Meshandin AG; Vaneeva LI; Agapkina NP; Mironova VV; Dargeeva TA
 SO    Zh Mikrobiol Epidemiol Immunobiol. 1993 Sep-Oct;(5):97-100. Unique
       Identifier : AIDSLINE MED/94346169
 AB    The practical aspects of the theory of the adsorption of bioligands on
       the plastic surface have been considered and discussed. The possibility
       of standardizing polystyrene plates, as well as obtaining plates with
       the predetermined level of adsorption activity with respect to given
       antigens (antibodies), has been shown with the use of the patented
       technology for the modification of polystyrene plates. The data on the
       adsorption of antigens used as an experimental model (human and rabbit
       immunoglobulin fractions) are presented, and an increase in adsorption
       activity has been shown with the use of modified plates. For practical
       confirmation, some examples of using these plates in actual assay
       systems intended for the evaluation of HBsAg carriership and antibodies
       to HIV are given. A considerable increase (2- to 10-fold) of the signal
       from positive samples has been shown to occur, the signal from negative
       samples remaining practically unchanged.
 DE    Adsorption  Animal  Antibodies, Viral/BLOOD  Communicable
       Diseases/*DIAGNOSIS  Comparative Study  English Abstract  Evaluation
       Studies  Hepatitis B Surface Antigens/BLOOD  Human  HIV Antibodies/BLOOD
       HIV-1/IMMUNOLOGY  IgG/BLOOD  Immunoenzyme Techniques/*INSTRUMENTATION
       Measles Virus/IMMUNOLOGY  *Polystyrenes  Rabbits  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).