Document 0553
 DOCN  M9490553
 TI    Reassessment of the differential effects of ultraviolet and ionizing
       radiation on HIV promoter: the use of cell survival as the basis for
       comparisons.
 DT    9411
 AU    Beer JZ; Olvey KM; Lee W; Zmudzka BZ; Center for Devices and
       Radiological Health, Food and Drug; Administration, Rockville, MD 20857.
 SO    Photochem Photobiol. 1994 Jun;59(6):643-9. Unique Identifier : AIDSLINE
       MED/94344998
 AB    Effects of different radiation treatments on the human immunodeficiency
       virus-1 (HIV) promoter were reassessed for exposures comparable to those
       encountered in clinical or cosmetic practice, using survival of the host
       cell as a basis for comparisons. The exposures were performed with two
       ultraviolet radiation sources commonly used as medical or cosmetic
       devices (UVASUN 2000 and FS20 lamps), a germicidal (G15T8) lamp and an
       X-ray machine. The UVC component of the FS20 lamp was filtered out. The
       emission spectra of the lamps were determined. The characteristics of
       these sources allowed us to discriminate among effects of UVA1 (340-400
       nm), UVB + UVA2 (280-340 nm) and UVC (254 nm) radiations. Effects of
       irradiation were ascertained using cultures of HeLa cells stably
       transfected with the HIV promoter linked to a reporter-chloramphenicol
       acetyl transferase-gene. The exposures used caused at least two logs of
       cell killing. In this cytotoxicity range, UVA1 or X radiations had no
       effect on the HIV promoter, whereas UVB + UVA2 or UVC radiations
       activated the HIV promoter in a fluence-dependent manner. Survivals
       following exposure to UVB + UVA2 or UVC radiation were (1) at the lowest
       measurable HIV promoter activation, 30 and 20%, respectively, (2) at
       one-half maximal activation, 6 and 3%, respectively and (3) at the
       maximal activation, 0.5 and 0.2%, respectively.(ABSTRACT TRUNCATED AT
       250 WORDS)
 DE    Cell Survival/RADIATION EFFECTS  Comparative Study  Gene Expression
       Regulation, Viral/RADIATION EFFECTS  Hela Cells  Human
       HIV-1/GENETICS/*RADIATION EFFECTS  Promoter Regions
       (Genetics)/*RADIATION EFFECTS  Support, U.S. Gov't, Non-P.H.S.  Support,
       U.S. Gov't, P.H.S.  Ultraviolet Rays  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).