Document 0608
 DOCN  M9490608
 TI    Detection of human T lymphotrophic virus type I (HTLV-I) proviral DNA
       and analysis of T cell receptor V beta CDR3 sequences in spinal cord
       lesions of HTLV-I-associated myelopathy/tropical spastic paraparesis.
 DT    9411
 AU    Hara H; Morita M; Iwaki T; Hatae T; Itoyama Y; Kitamoto T; Akizuki S;
       Goto I; Watanabe T; Department of Neurology, Faculty of Medicine, Kyushu
       University,; Fukuoka, Japan.
 SO    J Exp Med. 1994 Sep 1;180(3):831-9. Unique Identifier : AIDSLINE +
 AB    Identification of the localization of human T lymphotrophic virus type I
       (HTLV-I) proviral DNA in the central nervous system (CNS) is crucial to
       the understanding of the pathogenesis of HTLV-I-associated myelopathy
       (HAM)/tropical spastic paraparesis (TSP) pathogenesis. We have developed
       a sensitive detection method, called two-step polymerase chain reaction
       (PCR) in situ hybridization, which enabled us to detect the HTLV-I
       proviral DNA in paraffin-embedded spinal cord tissue sections from
       HAM/TSP patients. HTLV-I proviral DNA was detected only in the nucleus
       of lymphocytes that had infiltrated into the spinal cord. However, no
       proviral DNA was amplified in any neuronal cells, including neurons and
       glial cells. This indicates that the demyelination of the spinal cord by
       HTLV-I as a result of viral infection of oligodendrocytes or neuronal
       cells is unlikely. The T cell receptor V beta gene sequence from
       lymphocytes in the spinal cord lesions taken from the same HAM/TSP
       autopsy cases revealed unique and restricted CDR3 motifs, CASSLXG(G)
       (one-letter amino acid. X is any amino acid), CASSPT(G), and CASSGRL
       which are similar to those described in T cells from brain lesions of
       multiple sclerosis (MS) and in a rat T cell clone derived from
       experimental allergic encephalomyelitis (EAE) lesions. The present
       results suggest that T cells containing restricted V beta CDR3 motifs,
       which are also found in MS and EAE, become activated upon HTLV-I
       infection and infiltrate into the spinal cord lesions of HAM/TSP
       patients.
 DE    Adult  Aged  Amino Acid Sequence  Base Sequence  DNA, Viral/*ANALYSIS
       Female  Human  HTLV-I/*GENETICS  Lymphocyte Transformation  Male  Middle
       Age  Molecular Sequence Data  Paraparesis, Tropical
       Spastic/IMMUNOLOGY/*MICROBIOLOGY/PATHOLOGY  Proviruses/*GENETICS
       Receptors, Antigen, T-Cell, alpha-beta/*GENETICS  Spinal
       Cord/IMMUNOLOGY/*MICROBIOLOGY/PATHOLOGY  Support, Non-U.S. Gov't
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).