Document 0633
 DOCN  M9490633
 TI    Induced mouse spleen B-cell proliferation and secretion of
       immunoglobulin by lipid-associated membrane proteins of Mycoplasma
       fermentans incognitus and Mycoplasma penetrans.
 DT    9411
 AU    Feng SH; Lo SC; Department of Infectious and Parasitic Disease
       Pathology, Armed; Forces Institute of Pathology, Washington, D.C. 20306.
 SO    Infect Immun. 1994 Sep;62(9):3916-21. Unique Identifier : AIDSLINE
       MED/94341899
 AB    Mycoplasmas have been implicated as a possible cofactor in AIDS
       pathogenesis. Mycoplasma fermentans and M. penetrans infect human
       immunodeficiency virus-positive patients at a significantly higher
       frequency than non-human immunodeficiency virus-infected control
       subjects. Various mycoplasmal membrane preparations are known to affect
       the functions of immune cells both in vitro and in vivo. A group of
       lipid-associated membrane proteins (LAMPs) extracted by Triton X-114
       from mycoplasmas are major antigenic targets of human host antibody
       responses. In this study, LAMPs prepared from both M. fermentans and M.
       penetrans nonspecifically stimulated spleen cells of CBA/CaH mice to
       proliferate. LAMPs were also stimulatory to spleen cells from athymic
       mice. On the other hand, enriched splenic T cells from CBA/CaH mice with
       or without accessory cells responded poorly. Thus, the mitogenic effect
       of mycoplasmal LAMPs appeared mainly on B cells. High levels of
       immunoglobulin (Ig) M and low but detectable amounts of IgG were found
       in the supernatant of LAMP-treated splenic cell culture. M. penetrans
       LAMPs had a much more potent effect on murine spleen cells than did M.
       fermentans incognitus LAMPs in inducing both B-cell proliferation and Ig
       secretion. In conclusion, the mycoplasmal LAMPs contained an active
       component(s) with T-independent B-cell mitogenic effect.
 DE    Animal  B-Lymphocytes/*IMMUNOLOGY  Bacterial Proteins/*PHARMACOLOGY
       Female  Immunoblotting  Immunoglobulins/*BIOSYNTHESIS  Lymphocyte
       Transformation/*DRUG EFFECTS  Membrane Proteins/*PHARMACOLOGY  Mice
       Mice, Inbred CBA  Mycoplasma/*IMMUNOLOGY  Mycoplasma
       fermentans/*IMMUNOLOGY  Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).