Document 0656
 DOCN  M9490656
 TI    Conjugates of dendritic cells and memory T lymphocytes from skin
       facilitate productive infection with HIV-1.
 DT    9411
 AU    Pope M; Betjes MG; Romani N; Hirmand H; Cameron PU; Hoffman L; Gezelter
       S; Schuler G; Steinman RM; Laboratory of Cellular Physiology and
       Immunology Rockefeller; University, New York, New York 10021.
 SO    Cell. 1994 Aug 12;78(3):389-98. Unique Identifier : AIDSLINE
       MED/94340730
 AB    Experimentally, a productive infection with HIV-1 requires that virus be
       administered to T cells that are activated by mitogens. We describe a
       productive milieu for HIV-1 within the confines of normal skin that does
       not require standard stimuli. The milieu consists of dendritic cells and
       T cells that emigrate from skin and produce distinctive stable,
       nonproliferating conjugates. These conjugates, upon exposure to each of
       seven different HIV-1 isolates, begin to release high levels of virus
       progeny within 4 days. Numerous infected syncytia, comprised of both
       dendritic and T cells, rapidly develop. We propose that conjugates of
       dendritic cells and T cells, as found in the external linings of organs
       involved in sexual transmission of HIV-1, represent an important site
       for the productive phase of HIV-1 infection. Because the affected T
       cells carry the memory phenotype, this site additionally provides a
       mechanism for the chronic depletion of CD4+ memory cells in HIV-1
       disease.
 DE    Cell Fusion  Cell Movement  Dendritic Cells/IMMUNOLOGY/*MICROBIOLOGY
       Flow Cytometry  Giant Cells/*MICROBIOLOGY  Human  HIV Core Protein
       p24/ANALYSIS  HIV Infections/ETIOLOGY/IMMUNOLOGY/TRANSMISSION
       HIV-1/*GROWTH & DEVELOPMENT/IMMUNOLOGY  Immunologic Memory
       Skin/CYTOLOGY/IMMUNOLOGY/*MICROBIOLOGY  Support, Non-U.S. Gov't
       Support, U.S. Gov't, P.H.S.  T4 Lymphocytes/IMMUNOLOGY/*MICROBIOLOGY
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).