Document 0756
 DOCN  M9490756
 TI    Molecular epidemiology of HIV-1 in the former Soviet Union: analysis of
       env V3 sequences and their correlation with epidemiologic data.
 DT    9411
 AU    Bobkov A; Garaev MM; Rzhaninova A; Kaleebu P; Pitman R; Weber JN;
       Cheingsong-Popov R; Department of Molecular Virology, D.I. Ivanovsky
       Institute of; Virology, Academy of Medical Science, Moscow, Russia.
 SO    AIDS. 1994 May;8(5):619-24. Unique Identifier : AIDSLINE MED/94338597
 AB    OBJECTIVE: To investigate the HIV-1 V3 sequence diversity in the former
       Soviet Union in 30 subjects infected with HIV-1 via different modes of
       transmission. PATIENTS: A cohort of children infected after exposure to
       nonsterile needles during the epidemic in 1988-1989 in southern Russia
       (Elista, n = 12 and Rostov-on-Don, n = 10), and eight HIV-seropositive
       subjects from Belarus (Minsk), infected via sexual (n = 7) and
       parenteral (n = 1) infection. METHODS: The HIV-1 V3 encoding region was
       amplified by nested polymerase chain reaction on DNA of primary
       peripheral blood mononuclear cells collected from the study subjects and
       then cloned and sequenced. RESULTS: The alignment of 127 V3 sequences
       from 22 patients in the cohort group demonstrated common consensus
       sequences in both the Elista and Rostov samples. The average means of
       interperson variation were 5.9 and 6.6% in Elista and Rostov subjects,
       respectively, and comparable to the mean intraperson variation. The
       average mean interperson variation between nucleotide sequences of HIV
       patients infected through sexual transmission was considerably higher
       (14.9%). CONCLUSION: V3 sequence analysis confirms the epidemiologic
       data which support the transmission of HIV-1 in children from a single
       source, and suggests the infection of a mother from her parenterally
       infected child. Furthermore, the genetic variability of HIV-1 V3 in the
       noncohort group was particularly divergent indicating the heterogeneity
       of the virus circulating in the former Soviet Union.
 DE    Adolescence  Adult  Africa, Central  Base Sequence  Child  Child,
       Preschool  Cohort Studies  Comparative Study  Consensus Sequence  Cross
       Infection/*EPIDEMIOLOGY/MICROBIOLOGY/TRANSMISSION  *Disease Outbreaks
       Equipment Contamination  Female  *Genes, env  Genome, Viral  Human  HIV
       Envelope Protein gp120/*GENETICS  HIV
       Infections/CONGENITAL/*EPIDEMIOLOGY/MICROBIOLOGY/TRANSMISSION
       HIV-1/CLASSIFICATION/GENETICS/*ISOLATION & PURIF  Iatrogenic Disease
       Infant, Newborn  Injections, Intramuscular/ADVERSE EFFECTS  Injections,
       Intravenous/ADVERSE EFFECTS  Male  Molecular Sequence Data  *Needle
       Sharing/STATISTICS & NUMER DATA  Peptide Fragments/*GENETICS  Polymerase
       Chain Reaction  Pregnancy  Pregnancy Complications,
       Infectious/MICROBIOLOGY  Sequence Alignment  Sequence Homology, Nucleic
       Acid  Sex Behavior  Support, Non-U.S. Gov't  Travel  USSR/EPIDEMIOLOGY
       JOURNAL ARTICLE

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