Document 0761
 DOCN  M9490761
 TI    Characterization of a human Kaposi's sarcoma cell line that induces
       angiogenic tumors in animals.
 DT    9411
 AU    Herndier BG; Werner A; Arnstein P; Abbey NW; Demartis F; Cohen RL;
       Shuman MA; Levy JA; Department of Pathology, San Francisco General
       Hospital,; California.
 SO    AIDS. 1994 May;8(5):575-81. Unique Identifier : AIDSLINE MED/94338592
 AB    OBJECTIVE: To characterize a Kaposi's sarcoma (KS) cell line established
       from a tumor biopsy from the oral mucosa of an iatrogenically
       immunosuppressed HIV-negative man. METHODS: Cells were placed in culture
       and evaluated by a variety of biologic, serologic, karyotypic, and
       immunologic procedures. Electron microscopic examination was performed.
       The ability to produce tumors in nude mice was evaluated, and the nature
       of the cells within the tumor determined. Assays for urokinase
       plasminogen activator type (uPA), plasminogen activator inhibitor-1
       (PAI-1) and the urokinase receptor (uPAR) were conducted. RESULTS: The
       SLK cell line has an endothelial cell morphology with very little
       anaplasia. The karyotype indicates diploid phenotype of human origin.
       Immunohistochemical and electron microscopic examinations confirmed the
       endothelial nature of this cell line. No viruses were detected. The
       tumors induced in nude mice showed hypervascularization, with
       characteristics of KS. The cell line produces uPA and PAI-1, and also
       expresses uPAR. CONCLUSIONS: The SLK cell line is of endothelial cell
       origin and the first human cell line to induce KS-like tumors in
       recipient animals. The expression of urokinase and its receptor suggests
       a paracrine and autocrine interaction that may be important for the
       growth of the tumor. The SLK line should be valuable for studies of KS
       pathogenesis and therapeutic approaches to this malignancy.
 DE    Animal  Cell Division  Endothelium/PATHOLOGY  Human  HIV Seronegativity
       Immunocompromised Host  Karyotyping  Male  Mice  Mice, Nude  Mouth
       Mucosa/PATHOLOGY  Mouth Neoplasms/*PATHOLOGY  Neoplasm Proteins/ANALYSIS
       Neoplasm Transplantation  Neovascularization/*ETIOLOGY  Plasminogen
       Activator Inhibitor 1/ANALYSIS  Receptors, Cell Surface/ANALYSIS
       Sarcoma, Kaposi's/*PATHOLOGY  Support, U.S. Gov't, P.H.S.  *Tumor Cells,
       Cultured/PATHOLOGY/TRANSPLANTATION  Tumor Markers, Biological
       Urokinase/ANALYSIS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).