Document 0001
 DOCN  M94A0001
 TI    HIV coating gp 120 glycoprotein-dependent prostaglandin E2 release by
       human cultured astrocytoma cells is regulated by nitric oxide formation.
 DT    9412
 AU    Mollace V; Colasanti M; Rodino P; Lauro GM; Nistico G; Department of
       Biology, University of Rome Tor Vergata, Italy.
 SO    Biochem Biophys Res Commun. 1994 Aug 30;203(1):87-92. Unique Identifier
       : AIDSLINE MED/94354888
 AB    The role of the L-arginine-NO pathway on the formation of PGE2 by
       cultured astroglial cells incubated with the HIV coating glycoprotein
       gp120 was investigated. Preincubation of human cultured T 67 astrocytoma
       cells with gp 120 (100-500 nM) produced a significant increase of
       nitrite (the breakdown product of NO) and PGE2 in cell supernatants. The
       effect of gp 120 on both nitrite and PGE2 production was antagonized by
       inhibition of NO synthase by L-NAME (20-300 microM). The inhibition of
       gp120-induced PGE2 production by L-NAME was reverted by addition of
       arachidonic acid (30 microM), an effect antagonized by the
       cyclo-oxygenase inhibitor indomethacin (10 microM). Methylen bleu, an
       inhibitor of the biological activity of NO acting at the guanylate
       cyclase level failed to affect gp 120-mediated PGE2 release showing that
       the increase of cGMP subsequent to NO production was not involved in the
       modulatory activity of NO on arachidonic acid cascade. On the basis of
       present experiments we conclude that gp-120-induced release of PGE2 by
       astroglial cells is driven by NO, thereby contributing in the
       involvement of glial cells in HIV-related cerebral disorders.
 DE    Amino Acid Oxidoreductases/*ANTAGONISTS & INHIB  Arginine/*ANALOGS &
       DERIVATIVES/PHARMACOLOGY  Astrocytoma  Cell Line
       Dinoprostone/*METABOLISM  Dose-Response Relationship, Drug  Human  HIV
       Envelope Protein gp120/*PHARMACOLOGY  Indomethacin/PHARMACOLOGY
       Kinetics  Nitric Oxide/*BIOSYNTHESIS  Nitrites/METABOLISM
       Nitroprusside/PHARMACOLOGY  Support, Non-U.S. Gov't  Tumor Cells,
       Cultured  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).