Document 0002
 DOCN  M94A0002
 TI    Tumour necrosis factor alpha and interleukin-1 beta induce specific
       subunits of NFKB to bind the HIV-1 enhancer: characterisation of
       transcription factors controlling human immunodeficiency virus type 1
       gene expression in neural cells.
 DT    9412
 AU    Swingler S; Morris A; Easton A; Department of Biological Sciences,
       University of Warwick,; Coventry, UK.
 SO    Biochem Biophys Res Commun. 1994 Aug 30;203(1):623-30. Unique Identifier
       : AIDSLINE MED/94354868
 AB    In human astrocytoma and neuroblastoma cell lines tumour necrosis factor
       alpha (TNF alpha) and interleukin 1 beta (IL-1 beta) induced NFKB and an
       additional KB-specific protein of approximately 80 K to bind the HIV-1
       enhancer. One nucleoprotein complex contained prototypical NFKB
       comprising of p50 and p65 subunits and a second contained the p65
       subunit and an 80 K factor, p80. Over a 24 hr period of cytokine
       stimulation the p50/p65 complex of NFKB was present in the nucleus
       whilst the second complex declined in abundance after two hours due to
       the decline of p80. In unstimulated cells, DNAse I footprinting revealed
       a previously unidentified octamer-like binding site in the negative
       regulatory element (NRE) of the HIV-1 long terminal repeat (LTR) which
       specifically bound protein factors present in human astrocytoma,
       neuroblastoma and murine oligodendroglioma cell lines. A second unique
       motif, also in the NRE, was observed with extracts from one human
       neuroblastoma cell line and a murine oligodendroglioma. Footprinting
       analysis also confirmed that Sp1, TATA, Site A and Site B motifs of the
       LTR were occupied by nuclear factors present in neural cells.
 DE    Astrocytoma  Base Sequence  Binding Sites  Cell Line  Cell
       Nucleus/METABOLISM  Deoxyribonuclease I  DNA, Neoplasm/METABOLISM
       *Enhancer Elements (Genetics)  Human  HIV-1/*GENETICS/METABOLISM
       Interleukin-1/*PHARMACOLOGY  Macromolecular Systems  Molecular Sequence
       Data  Neuroblastoma  Neurons/METABOLISM  NF-kappa B/*METABOLISM
       Oligodeoxyribonucleotides  Support, Non-U.S. Gov't  Transcription
       Factors/*METABOLISM  Tumor Cells, Cultured  Tumor Necrosis
       Factor/*PHARMACOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).