Document 0018
 DOCN  M94A0018
 TI    Inactivation of free and cell-associated human immunodeficiency virus in
       platelet suspensions by aminomethyltrimethylpsoralen and ultraviolet
       light.
 DT    9412
 AU    Benade LE; Shumaker J; Xu Y; Chen X; Dodd RY; Jerome H. Holland
       Laboratory for the Biomedical Sciences,; American Red Cross, Rockville,
       Maryland.
 SO    Transfusion. 1994 Aug;34(8):680-4. Unique Identifier : AIDSLINE
       MED/94353463
 AB    BACKGROUND: It has previously been reported that 40 micrograms per mL of
       aminomethyltrimethylpsoralen (AMT) plus 2.4 to 7.2 J per cm2 of
       ultraviolet A (UVA) light inactivated 4 to 6 log10 of several model
       viruses in platelet suspensions. This inactivation was achieved while
       satisfactory levels of platelet count, pH, morphology, aggregation, and
       hemostatic effectiveness were maintained. STUDY DESIGN AND METHODS: The
       efficacy of this procedure for inactivating free and intracellular human
       immunodeficiency virus (HIV), including integrated proviral sequences,
       was studied. RESULTS: The kinetics of inactivation for free HIV (4-5
       log10 kill with 1.2-4.8 J/cm2) were similar to those obtained for the
       previously studied viruses. For studies on cell-associated virus, H9
       cells productively infected with HIV were added to platelet suspensions
       and treated with the above regimen of AMT and UVA. The phototreated
       cells were then cocultivated with uninfected H9 cells for 4 weeks and
       supernatants were assayed by enzyme-linked immunosorbent assay for HIV
       p24. No evidence of HIV replication was detectable for cells receiving
       as little as 2.4 J per cm2 of UVA irradiation in the presence of AMT.
       Further, it has been demonstrated that stably integrated sequences from
       the HIV proviral env gene can no longer be amplified by polymerase chain
       reaction after 1.2 J per cm2 of UVA (with 40 micrograms/mL AMT)
       exposure. CONCLUSION: These data suggest that AMT and UVA is an
       effective antiviral treatment for free and cell-associated HIV in
       platelet suspensions.
 DE    Base Sequence  Blood Platelets/*MICROBIOLOGY  DNA, Viral/DRUG
       EFFECTS/RADIATION EFFECTS  Human  HIV/*DRUG EFFECTS/*RADIATION EFFECTS
       Molecular Sequence Data  Photochemistry  Polymerase Chain Reaction
       Proviruses/DRUG EFFECTS/RADIATION EFFECTS  Sindbis Virus/DRUG
       EFFECTS/RADIATION EFFECTS  Trioxsalen/*ANALOGS &
       DERIVATIVES/PHARMACOLOGY  *Ultraviolet Rays  Vesicular
       Stomatitis-Indiana Virus/DRUG EFFECTS/RADIATION EFFECTS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).