Document 0032
 DOCN  M94A0032
 TI    [The evaluation of the effect of formol and sodium hypochlorite on the
       demonstration of Cryptosporidium oocysts in feces by Heine's method]
 DT    9412
 AU    Modolo JR; Amato Neto V; Braz LM; Lopes CA; Gottschalk AF; Faculdade de
       Medicina Veterinaria e Zootecnia de Botucatu,; Universidade Estadual
       Paulista, SP, Brazil.
 SO    Rev Soc Bras Med Trop. 1994 Apr-Jun;27(2):75-7. Unique Identifier :
       AIDSLINE MED/94353093
 AB    Cryptosporidium oocysts were searched by Heine's method in stools of
       nine calves with cryptosporidiosis after stool treatment with two
       disinfectants, 10% paraformaldehyde solution and 14.5% sodium
       hypochlorite solution. After 30 minutes exposition to sodium
       hypochlorite solution oocysts became non refractile and acquired a
       reddish tinge, making their identification difficult. No morphological
       alterations occurred in oocysts after paraformaldehyde treatment. We
       recommend paraformaldehyde at 10% concentration as means of human
       immunodeficiency virus (HIV) inactivation for routine use in stool
       examinations and therefore making safer those type of procedures for
       laboratory personnel, when using Heine's method.
 DE    Animal  AIDS-Related Opportunistic Infections/PARASITOLOGY  Cattle
       Cattle Diseases/PARASITOLOGY  Comparative Study
       Cryptosporidiosis/PARASITOLOGY  Cryptosporidium/*DRUG EFFECTS/ISOLATION
       & PURIF  English Abstract  Evaluation Studies  Feces/*PARASITOLOGY
       Formaldehyde/*PHARMACOLOGY  Human  HIV-1  Indicators and Reagents
       Methods  Sodium Hypochlorite/*PHARMACOLOGY  JOURNAL ARTICLE

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