Document 0095
 DOCN  M94A0095
 TI    In vitro susceptibility and sterol biosynthesis of Candida albicans
       strains after long-term treatment with azoles in HIV-infected patients.
 DT    9412
 AU    Hundt W; Hofmann H; Dermatologische Klinik und Poliklinik, Technischen
       Universitat,; Munchen, Germany.
 SO    Infection. 1994 Mar-Apr;22(2):124-31. Unique Identifier : AIDSLINE
       MED/94350501
 AB    Over a period of 6 to 24 months a long term follow up of the in vitro
       antifungal susceptibility of 306 Candida albicans strains from 49
       HIV-infected patients was performed. Using a microdilution test, the
       strains were tested against the azoles ketoconazole, itraconazole and
       fluconazole. The susceptibility range for fluconazole was between 1 and
       128 mg/l, for itraconazole between 0.015 and 32 mg/l and for
       ketoconazole between 0.007 and 16 mg/l. 11.7% of the strains showed
       elevated IC30-values against ketoconazole, 9.1% against itraconazole and
       10.1% against fluconazole. Sterol biosynthesis was examined by thin
       layer chromatography in 18 less sensitive strains and nine sensitive
       strains in the presence of ketoconazole. The proportion of ergosterol in
       the presence of ketoconazole at a concentration of 0.003 mg/l varied
       between 7.6% and 21.1% in sensitive strains and between 11.1% and 86.6%
       in less sensitive strains. In resistant control strains the proportion
       of ergosterol was 73% and 94.2%, respectively. Without ketoconazole the
       ergosterol proportion was > 85% in all strains. There was a significant
       correlation between the IC30-values and the inhibition of ergosterol
       biosynthesis (p = 0.05).
 DE    AIDS-Related Opportunistic Infections/DRUG THERAPY/*MICROBIOLOGY
       Candida albicans/*DRUG EFFECTS/GROWTH & DEVELOPMENT  Candidiasis,
       Oral/DRUG THERAPY/*MICROBIOLOGY  Chromatography, Thin Layer  Drug
       Resistance, Microbial  Evaluation Studies
       Fluconazole/PHARMACOLOGY/*THERAPEUTIC USE  Follow-Up Studies  Human
       Itraconazole/PHARMACOLOGY/*THERAPEUTIC USE
       Ketoconazole/PHARMACOLOGY/*THERAPEUTIC USE  Microbial Sensitivity Tests
       Sterols/*BIOSYNTHESIS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).