Document 0165
 DOCN  M94A0165
 TI    Epitope mapping and topology of baculovirus-expressed HIV-1 gp160
       determined with a panel of murine monoclonal antibodies.
 DT    9412
 AU    Abacioglu YH; Fouts TR; Laman JD; Claassen E; Pincus SH; Moore JP; Roby
       CA; Kamin-Lewis R; Lewis GK; Department of Microbiology and Immunology,
       University of Maryland; School of Medicine, Baltimore 21201.
 SO    AIDS Res Hum Retroviruses. 1994 Apr;10(4):371-81. Unique Identifier :
       AIDSLINE MED/94347461
 AB    To define protein folding patterns of HIV-1 Env subunit vaccines, we
       have isolated a set of 30 monoclonal antibodies (MAbs) from BALB/c mice
       immunized with a recombinant gp160 vaccine (rgp160) expressed in a
       baculovirus system. This article describes epitope mapping for the MAb
       panel and topology of the epitopes for rgp160 and a recombinant gp120
       (rgp120) also expressed in a baculovirus system. The following results
       are reported: (1) rgp160 harbors a minimum of 4 antigenic domains, 3
       mapping to the C1, C2, and C3/V4 regions of gp120 and 1 mapping to the
       cytoplasmic tail of gp41; (2) there are at least 3 adjacent or
       overlapping epitopes in each antigenic domain; (3) a minimum of 14
       independent epitopes were mapped, all of which are continuous sites; (4)
       each of the epitopes is exposed on rgp160 without prior manipulation of
       the protein; and (5) by contrast, 6 of the 8 epitopes mapping to the C1,
       C2, and C3/V4 regions are not exposed on rgp120, but become exposed when
       the protein is denatured. Taken together, these results show that rgp160
       and rgp120 are folded differently, illustrating the use of this MAb
       panel to compare epitope topographies of recombination HIV-1 Env
       proteins. This MAb panel may aid in the refinement of HIV-1 Env subunit
       vaccines.
 DE    Amino Acid Sequence  Animal  Antibodies, Monoclonal  AIDS
       Vaccines/CHEMISTRY/IMMUNOLOGY  Baculoviridae/GENETICS  Enzyme-Linked
       Immunosorbent Assay  Female  Gene Products,
       env/CHEMISTRY/*GENETICS/*IMMUNOLOGY  HIV Antigens/CHEMISTRY/*GENETICS
       HIV Envelope Protein gp120/CHEMISTRY/GENETICS/IMMUNOLOGY
       HIV-1/*GENETICS/*IMMUNOLOGY  Immunization  Mice  Mice, Inbred BALB C
       Molecular Sequence Data  Peptide Mapping  Protein Folding  Protein
       Precursors/CHEMISTRY/*GENETICS/*IMMUNOLOGY  Sequence Deletion  Support,
       Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).