Document 0222
 DOCN  M94A0222
 TI    Increased spontaneous secretion of interleukin 6 and tumor necrosis
       factor alpha by peripheral blood lymphocytes of human immunodeficiency
       virus-infected children.
 DT    9412
 AU    Gurram M; Chirmule N; Wang XP; Ponugoti N; Pahwa S; Department of
       Pediatrics, North Shore University Hospital,; Cornell University Medical
       College, Manhasset NY 11030.
 SO    Pediatr Infect Dis J. 1994 Jun;13(6):496-501. Unique Identifier :
       AIDSLINE MED/94359772
 AB    Interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-alpha) have
       been implicated in the transition of nonreplicating latent human
       immunodeficiency virus (HIV) infection to the replicating state of
       productive infection. In HIV infection increased concentrations of these
       cytokines in serum have also been found in association with
       hypergammaglobulinemia. We have analyzed the ability of peripheral blood
       mononuclear cells (PBMC) of HIV-infected children to secrete IL-6 and
       TNF-alpha. In kinetic studies, optimum spontaneous IL-6 secretion by 1 x
       10(6) PBMC was achieved at 24 hours. The mean spontaneous IL-6 and
       TNF-alpha concentrations secreted by PBMC of known HIV-infected children
       (age range, 8 months to 11 years) were 1686 and 131 pg/ml, respectively,
       compared with 56 and 45 pg/ml, respectively, in normal healthy controls.
       No significant correlation was observed between spontaneously secreted
       IL-6 and TNF-alpha in culture supernatants with CD4 or CD8 numbers; with
       serum IgG, IgA and IgM concentrations; or with lymphoproliferative
       responses to recall antigens. There was, however, an association between
       ability to secrete IL-6 with HIV-specific in vitro antibody production.
       Spontaneous IL-6 secretion decreased transiently after initiation of
       antiretroviral therapy, returning to original values with continued
       treatment. Cytokine derangement in HIV-infected children includes
       PBMC-derived spontaneous IL-6 and TNF-alpha secretion.
 DE    Child  Child, Preschool  Female  Human  HIV Antibodies/BIOSYNTHESIS  HIV
       Infections/*BLOOD/DRUG THERAPY/METABOLISM  Infant
       Interleukin-6/BIOSYNTHESIS/*SECRETION  Leukocytes,
       Mononuclear/IMMUNOLOGY/*SECRETION  Male  Pilot Projects  Support, U.S.
       Gov't, P.H.S.  Tumor Necrosis Factor/BIOSYNTHESIS/*SECRETION
       Zidovudine/THERAPEUTIC USE  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).