Document 0248
 DOCN  M94A0248
 TI    Growth ability of human immunodeficiency virus type 1 auxiliary gene
       mutants in primary blood macrophage cultures.
 DT    9412
 AU    Kawamura M; Ishizaki T; Ishimoto A; Shioda T; Kitamura T; Adachi A;
       Department of Viral Oncology, Kyoto University, Japan.
 SO    J Gen Virol. 1994 Sep;75 ( Pt 9):2427-31. Unique Identifier : AIDSLINE
       MED/94358745
 AB    A strain of human immunodeficiency virus type 1 that is strictly tropic
       for primary human blood cell cultures was constructed in vitro.
       Mutational studies on the vif, vpr, vpu and nef genes of this virus were
       performed to evaluate their biological functions in natural target
       cells. For this purpose, replication properties of mutant viruses in
       peripheral blood mononuclear cells (PBMCs) and macrophages (PBMPs) were
       determined. Three phenotypes with respect to virus replication were
       noticed: normal or mildly retarded growth (nef and vpr mutants),
       impaired growth (vpu mutant), and no growth (vif mutant). These results
       suggest that the Vif and Vpu proteins are more important than the Nef
       and Vpr proteins for virus replication in PBMCs and PBMPs.
 DE    Cell Line  Cells, Cultured  Comparative Study  *Genes, Structural, Viral
       Genome, Viral  Human  HIV Long Terminal Repeat  HIV-1/GROWTH &
       DEVELOPMENT/GENETICS/*PHYSIOLOGY  Kinetics  Leukemia, Promyelocytic,
       Acute  Lymphocytes/MICROBIOLOGY  Macrophages/*MICROBIOLOGY  *Mutation
       Restriction Mapping  Support, Non-U.S. Gov't  Virus
       Replication/*GENETICS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).