Document 0249
 DOCN  M94A0249
 TI    Human cytomegalovirus late protein encoded by ie2: a trans-activator as
       well as a repressor of gene expression.
 DT    9412
 AU    Jenkins DE; Martens CL; Mocarski ES; Department of Microbiology and
       Immunology, Stanford University; School of Medicine, California
       94305-5402.
 SO    J Gen Virol. 1994 Sep;75 ( Pt 9):2337-48. Unique Identifier : AIDSLINE
       MED/94358733
 AB    In order to study the function of human cytomegalovirus (HCMV) immediate
       early gene 2 (ie2) (UL122) gene products made at late times during
       infection, cDNA clones were isolated from an expression library made
       with 74 h post-infection mRNA. Based on screening of the library, 1% of
       transcripts in infected cells at this time were ie2 region-specific, and
       transcripts encoding gamma IE2(338aa), a 40K late gene product, were
       more abundant than those encoding IE2(579aa), an alpha gene product made
       throughout infection. As expected, the cDNA capable of directing the
       expression of gamma IE2(338aa) was derived from a contiguous genomic
       region within exon 5 of the ie1/ie2 region. The cDNA clones encoding
       gamma IE2(338aa) and IE2(579aa) were compared for their ability to
       trans-activate viral and cellular promoters and to repress expression
       from the ie1/ie2 promoter via the ie2 cis-repression signal.
       Unexpectedly, gamma IE2(338aa) trans-activated a variety of test
       promoters when cotransfected with the major alpha gene product,
       IE1(491aa). Promoters derived from the cellular beta-actin gene, the
       simian virus 40 early region and the human immunodeficiency virus were
       all responsive to gamma IE2(338aa) plus IE1(491aa), although several
       beta promoters derived from the HCMV genome were unresponsive. Thus,
       this abundant late product from the ie2 region may play a role in
       trans-activation in addition to its role as a repressor of alpha gene
       expression.
 DE    Animal  Base Sequence  Cell Line  Cells, Cultured  Cercopithecus
       aethiops  Chloramphenicol Acetyltransferase/BIOSYNTHESIS  Cloning,
       Molecular  Cytomegalovirus/GENETICS/*METABOLISM  DNA Primers  DNA,
       Complementary/METABOLISM  Fibroblasts  *Gene Expression Regulation,
       Viral  Gene Library  *Genes, Immediate-Early  *Genes, Viral  Human
       Immediate-Early Proteins/GENETICS/*METABOLISM  Male  Molecular Sequence
       Data  Plasmids  Polymerase Chain Reaction  Restriction Mapping  RNA,
       Messenger/BIOSYNTHESIS  Skin  Support, U.S. Gov't, P.H.S.
       *Trans-Activation (Genetics)  Trans-Activators/GENETICS/*METABOLISM
       Transfection  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).