Document 0260
 DOCN  M94A0260
 TI    Treatment of human immunodeficiency virus-infected lymphocytes with
       cationized human immunoglobulins.
 DT    9412
 AU    Pardridge WM; Buciak J; Yang J; Diagne A; Department of Medicine,
       University of California, School of; Medicine, Los Angeles 90024.
 SO    J Infect Dis. 1994 Sep;170(3):563-9. Unique Identifier : AIDSLINE
       MED/94358488
 AB    Hyperimmune immunoglobulins from human immunodeficiency virus
       (HIV)-infected persons may inhibit intracellular viral replication if
       the HIV immunoglobulins (HIVIG) are delivered to intracellular spaces
       where the virus replicates. This study examines the hypothesis that
       cationized forms of HIVIG undergo enhanced absorptive-mediated
       endocytosis into cells and inhibit HIV replication. HIVIG and nonimmune
       human intravenous immunoglobulin (IVIG) were cationized with
       hexamethylenediamine to an isoelectric point (pI) > 9.5. Cationization
       markedly increased the binding and endocytosis of HIVIG and IVIG by
       human peripheral blood lymphocytes (PBL). Cationized HIVIG and IVIG (250
       micrograms/mL) resulted in a 90% inhibition in p24 released to the
       medium in HIV-1-infected human PBL. The study demonstrates that
       cationization of human immunoglobulin preparations, such as HIVIG and
       IVIG, markedly increases the endocytosis of immunoglobulin and the
       inhibition of HIV-1 replication in human PBL.
 DE    Cations  Diamines  DNA/BIOSYNTHESIS  Electrophoresis, Polyacrylamide Gel
       Endocytosis  Human  HIV-1/DRUG EFFECTS/IMMUNOLOGY/*PHYSIOLOGY
       Immunoglobulins, Intravenous/ISOLATION & PURIF/METABOLISM/
       *PHARMACOLOGY  Iodine Radioisotopes  Isoelectric Focusing  Kinetics
       Lymphocytes/IMMUNOLOGY/*MICROBIOLOGY  Support, U.S. Gov't, P.H.S.
       Thymidine/METABOLISM  Tritium  Virus Replication/*DRUG EFFECTS  JOURNAL
       ARTICLE

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