Document 0294
 DOCN  M94A0294
 TI    Sodium benzylideneascorbate induces apoptosis in HIV-replicating U1
       cells.
 DT    9412
 AU    Aoki K; Nakashima H; Hattori T; Shiokawa D; Ni-imi E; Tanimoto Y; Maruta
       H; Uchiumi F; Kochi M; Yamamoto N; et al; Department of Biochemistry,
       Faculty of Pharmaceutical Sciences,; Science University of Tokyo, Japan.
 SO    FEBS Lett. 1994 Aug 29;351(1):105-8. Unique Identifier : AIDSLINE
       MED/94357260
 AB    U1 cells, a subclone of U937 cells chronically infected with human
       immunodeficiency virus type 1 (HIV-1), produced HIV-1 only in the
       presence of inducers such as 12-O-tetradecanoxylphorbol 13-acetate (TPA)
       or tumor necrosis factor (TNF)-alpha. The expression of HIV-antigen on
       U1 cells induced by TPA or TNF-alpha was found to be prevented by sodium
       5,6-benzylidene-L-ascorbate (SBA) in a concentration-dependent manner.
       Treatment of U1 cells with SBA in the presence of inducers resulted in
       cell death with cell shrinkage, chromatin condensation and DNA
       fragmentation into nucleosomal oligomers, characteristics of apoptosis.
       In contrast, SBA had scarcely any apoptotic effect on U1 cells in the
       absence of inducers. SBA did not also induce apoptosis in parental U937
       cells in the presence or absence of inducers. These results suggest that
       HIV-replicating U1 cells selectively undergo apoptosis on treatment with
       SBA.
 DE    Antiviral Agents/*PHARMACOLOGY  Apoptosis/*DRUG EFFECTS  Ascorbic
       Acid/*ANALOGS & DERIVATIVES/PHARMACOLOGY  Benzylidene
       Compounds/*PHARMACOLOGY  Human  HIV Antigens/ANALYSIS  HIV-1/*DRUG
       EFFECTS  Molecular Structure  Support, Non-U.S. Gov't
       Tetradecanoylphorbol Acetate/PHARMACOLOGY  Tumor Cells, Cultured  Tumor
       Necrosis Factor/PHARMACOLOGY  Virus Replication/DRUG EFFECTS  JOURNAL
       ARTICLE

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