Document 0586
 DOCN  M94A0586
 TI    Induction of pro-inflammatory cytokines by Cryptococcus neoformans.
 DT    9412
 AU    Visvanathan K; Jones PD; Department of Infectious Diseases, Prince Henry
       Hospital, Sydney,; Australia.
 SO    Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:93 (poster no. 36).
       Unique Identifier : AIDSLINE ASHM5/94349069
 AB    AIM: To characterise the induction of proinflammatory cytokines by C.
       neoformans and to determine if complement is an essential factor in this
       induction. METHODS: Peripheral blood mononuclear cells (PBM) were
       obtained from normal hosts and stimulated in vitro with either killed,
       whole C. neoformans or lipopolysaccharide. All steps in the in vitro
       assays were performed under conditions to minimise endotoxin
       contamination. RESULTS: Experiments showed that peak levels of IL-1
       beta, B, TNF-alpha and IL-6 were detected when first measured at 10 hrs
       of stimulation with C. neoformans. IL-1 beta and TNF-alpha levels
       declined after 24 hrs whereas IL-6 production persisted up to 9 days.
       The kinetics and magnitude of responses to C. neoformans were similar to
       LPS. Experiments showed that lower concentrations of cryptococcus had
       similar TNF-alpha production with both heat inactivated and normal sera,
       but was decreased in the absence of sera. However, at higher
       concentrations the TNF-alpha production was independent of the presence
       of sera. CONCLUSIONS: C. neoformans induces the production of
       proinflammatory cytokines with similar kinetics to LPS. The induction of
       TNF-alpha was independent of complement.
 DE    Cells, Cultured  Complement/PHYSIOLOGY  Cryptococcosis/*IMMUNOLOGY
       Cryptococcus neoformans/*IMMUNOLOGY  Cytokines/*BLOOD  Human
       Monocytes/IMMUNOLOGY  Reference Values  Tumor Necrosis Factor/METABOLISM
       MEETING ABSTRACT

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