Document 0621
 DOCN  M94A0621
 TI    Production of HIV DNA, mRNA and protein without infectious virus in
       growth-arrested cells.
 DT    9412
 AU    Kiernan R; Purcell D; McPhee D; National Centre in HIV Virology
       Research, Cellular Biology Unit,; Macfarlane Burnet Centre for Medical
       Research, Fairfield, Vic.
 SO    Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:73 (abstract no. SB6).
       Unique Identifier : AIDSLINE ASHM5/94349034
 AB    We have previously shown that an HTLV-I transformed cell line, MT-2,
       arrested in cell division and proliferation by treatment with 5 mM
       hydroxyurea could not be productively infected by HIV. Both release of
       cell-free virus and cell-cell fusion were found to be inhibited.
       Step-wise analyses of HIV replication have shown that complete reverse
       transcripts are synthesized in growth arrested cells. Nuclear
       translocation of viral DNA and subsequent production of both 1 and 2 LTR
       circular forms were found to occur. However, integration was
       significantly delayed. The expected range of spliced mRNAs was
       detectable despite lack of viral integration. Furthermore, viral protein
       could also be detected in the absence of integration. This data provides
       further evidence for the role of unintegrated HIV DNA as a competent
       template for transcription and translation of viral proteins. It also
       suggests that integration of viral DNA is required for efficient virus
       production since the presence of viral protein and RNA is insufficient
       for particle formation. We are currently addressing the role of viral
       and cellular factors in viral morphogenesis and cell-cell fusion in
       growth arrested cells.
 DE    Cell Division/*GENETICS  Cells, Cultured  DNA, Viral/*GENETICS  Gene
       Expression Regulation, Viral/PHYSIOLOGY  Human
       HIV/*GENETICS/PATHOGENICITY  HIV Antigens/*GENETICS  Repetitive
       Sequences, Nucleic Acid/GENETICS  RNA Splicing/GENETICS  RNA,
       Messenger/*GENETICS  Virulence/GENETICS  Virus Replication/*GENETICS
       MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).