Document 0622
 DOCN  M94A0622
 TI    Effects of GMCSF and HIV-1 infection on the expression of CD11a on
       monocyte-derived macrophages.
 DT    9412
 AU    Stent G; Crowe SM; Macfarlane Burnet Centre for Medical Research,
       Fairfield,; Australia.
 SO    Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:73 (abstract no. SB5).
       Unique Identifier : AIDSLINE ASHM5/94349033
 AB    OBJECTIVES: To examine the kinetics and regulation of CD11a on monocytes
       (MO) and macrophages (Mphi). METHODS: Mphi were isolated from buffy
       coats (n = 4) by density gradient centrifugation and plastic adherence
       or elutriation. (1) MO were incubated with varying concentrations of
       GMCSF (0-500U/ml) on the day of isolation and surface stained with CD11a
       MAb (DAKO) at regular intervals. (2) Mphi were infected with HIV-1 (BaL)
       at 5d post isolation and stained weekly with anti-CD11a. HIV-1 p24 Ag
       expression was also determined by FACS. Percent positive cells and
       equivalent molecules of fluorescence (EMF) as determined by calibration
       beads, were quantitated by flow cytometry. RESULTS: (1) GMCSF
       upregulated CD11a expression on Mphi in a dose dependent manner: 500U/ml
       GMCSF, CD11a 91% staining (EMF 240,000.); no cytokine, CD11a 79% (EMF
       92,000). (2) HIV-1 upregulated expression of CD11a: infected, CD11a 91%
       +/- 35 (EMF 283,000 +/- 105,000); uninfected, CD11a 83% +/- 5 (EMF
       191,000 +/- 76,000). CONCLUSION: Both GMCSF and HIV-1 appear to
       upregulate surface expression of CD11a on Mphi. HIV may stimulate MO to
       produce GMCSF which could in turn upregulate CD11a expression. This is
       being investigated.
 DE    Cell Line  Gene Expression Regulation, Viral/DRUG EFFECTS
       Granulocyte-Macrophage Colony-Stimulating Factor/*PHARMACOLOGY  Human
       HIV-1/*GENETICS  Lymphocyte Function-Associated Antigen-1/*GENETICS
       Macrophages/IMMUNOLOGY/*MICROBIOLOGY  Monocytes/IMMUNOLOGY/*MICROBIOLOGY
       Up-Regulation (Physiology)/GENETICS  Virus Integration/*DRUG
       EFFECTS/GENETICS  Virus Replication/*DRUG EFFECTS/GENETICS  MEETING
       ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).