Document 0623
 DOCN  M94A0623
 TI    Surface protein analysis of a non-syncytium inducing HIV-1 isolate.
 DT    9412
 AU    Fryer KM; Deacon NJ; Land SA; McPhee DA; Macfarlane Burnet Centre for
       Medical Research, Fairfield, Vic.
 SO    Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:72 (abstract no. SB3).
       Unique Identifier : AIDSLINE ASHM5/94349032
 AB    An Australian HIV-1 isolate that is non-syncytium inducing (NSI), and
       has reduced cytopathic effect on peripheral blood mononuclear cells
       (PBMCs) has been characterised. The biochemical properties and DNA
       sequence of this isolate (243925) were compared with a typical syncytium
       inducing (SI) isolate 228200. Both isolates replicate well in PBMCs and
       are neutralised by similar concentrations of sCD4 and OKT4a, but the NSI
       isolate does not down-modulate CD4 from the surface of infected cells.
       Phylogeny analysis based on envelope protein DNA sequence has mapped
       both to clade B, with North American isolates. The nucleotide sequences
       of the V3 loop of envelope of the two viruses correlate with published
       syncytium and non-syncytium inducing virus sequences (Fouchier et al.,
       1992). Western blot analysis has demonstrated greater heterogeneity in
       the envelope protein on the surface of cells infected with 243925
       compared with infection by the NSI isolate. The surface glycoprotein gp
       120 was detected on the viral particles of the NSI isolate only,
       suggesting that the protein is shed from the surface of the SI isolate.
       These studies confirm the similarity of Australian HIV-1 isolates to
       American isolates, and suggest a role for the stability of the envelope
       protein in HIV-1 syncytium formation.
 DE    Australia  Cell Line  Cytopathogenic Effect, Viral/GENETICS  DNA,
       Viral/*GENETICS  Gene Expression Regulation, Viral/PHYSIOLOGY  Giant
       Cells/*MICROBIOLOGY  Human  HIV Envelope Protein gp120/GENETICS
       HIV-1/*GENETICS  Monocytes/*MICROBIOLOGY  T4 Lymphocytes/MICROBIOLOGY
       MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).