Document 0626
 DOCN  M94A0626
 TI    Analysis of HIV-induced apoptosis: viral infection is required.
 DT    9412
 AU    Corbeil J; Howell ML; Richman DD; University of California San Diego,
       Department of Pathology, La; Jolla 92093-0679.
 SO    Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:71 (abstract no. SB1).
       Unique Identifier : AIDSLINE ASHM5/94349029
 AB    Lymphoblastoid T cell lines (SupTl, MT-2 and WE 17/10) and preparations
       of peripheral blood lymphocytes (CD4+ T cells enriched to 90%) were
       exposed to HIV-1LAI either untreated or inactivated with
       4'-aminomethyltrioxsalen (10 approximately g/mL) in combination with
       long-wave-length ultraviolet light (320-400nm) to render the viral
       preparation noninfectious while preserving its antigenicity. Apoptosis
       was quantified using fluorescence activated flow cytometry using the
       intercalative drug propidium iodine (PI) and further confirmed by the
       presence of characteristic nuclear condensation and digestion of host
       cell DNA. Apoptosis occurred in 37% of the cells over a 3 days period
       with the infectious HIV-1 stock but not with the treated virus.
       Cytopathicity due to syncytium formation accounted for only 6% of cell
       death registered during that period. Cell death was proportional to the
       amount of viral inoculum. This indicates that cell surface signalling by
       the virus alone is insufficient to trigger apoptosis but when the virus
       has entered the cell it can induce apoptosis.
 DE    Apoptosis/*GENETICS  Cell Line, Transformed  Flow Cytometry  Human
       HIV-1/*GENETICS/PATHOGENICITY  Signal Transduction/GENETICS  T4
       Lymphocytes/*MICROBIOLOGY  Virulence/GENETICS  Virus
       Integration/*GENETICS  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).