Document 0627
 DOCN  M94A0627
 TI    Reverse transcription factories in cell-to-cell HIV infection.
 DT    9412
 AU    Karageorgos LE; Li P; Burrell CJ; National Centre for HIV Virology
       Research, Institute of Medical; and Veterinary Science, Adelaide, South
       Australia.
 SO    Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:70 (abstract no. FB6).
       Unique Identifier : AIDSLINE ASHM5/94349028
 AB    Reverse transcription, the conversion of the single-stranded RNA genome
       into double-stranded DNA molecule, is a major event in the life cycle of
       retroviruses. We have shown that full-length unintegrated viral DNA in
       the cytoplasm of HIV infected cells is found in association with the
       viral proteins reverse transcriptase, integrase, matrix protein p17,
       protease and cellular histones, in a 320S replication complex. Using a
       one-step cell-to-cell transmission infection model, the stages of
       reverse transcription in HIV infection were investigated. The minus
       strong-stop and the first template transfer were detected as early as
       1.5 hours after infection, with completion of the full-length
       double-stranded DNA molecule by 3.5 hours, as detected by PCR. Evidence
       suggests that reverse transcription can proceed from initiation to
       completion within the 320s nucleoprotein complex.
 DE    DNA Nucleotidyltransferases/GENETICS  Gene Expression Regulation,
       Viral/PHYSIOLOGY  Gene Products, gag/GENETICS  Human  HIV
       Antigens/GENETICS  HIV Infections/*MICROBIOLOGY  HIV-1/*GENETICS
       Polymerase Chain Reaction  Reverse Transcriptase/*GENETICS
       Transcription, Genetic/*GENETICS  Virus Integration/*GENETICS  Virus
       Replication/GENETICS  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).