Document 0628
 DOCN  M94A0628
 TI    Double-stranded strong-stop DNA and HIV reverse transcription.
 DT    9412
 AU    Li P; Stephenson A; Kuiper L; Burrell CJ; National Centre for HIV
       Virology Research, Institute of Medical; and Veterinary Science,
       Adelaide, South Australia.
 SO    Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:70 (abstract no. FB7).
       Unique Identifier : AIDSLINE ASHM5/94349027
 AB    Using a synchronized, one-step HIV infection model, we report here the
       detection in acutely infected cells of a novel double-stranded
       strong-stop HIV DNA with a discrete length of approximately 650 base
       pairs, commencing at or near the left hand end of the right hand U3
       region of the HIV long terminal repeat. The plus-strand of this
       double-stranded strong-stop DNA possesses the primer binding site
       sequence and appears to be synthesized prior to the completion of the
       synthesis of its complementary minus-strand. In contrast, the
       minus-strand of the double-stranded strong-stop DNA lacks the primer
       binding site sequence after RNaseH digestion. We propose that a
       transient free plus-strand strong-stop DNA is released from its template
       by displacement synthesis and subsequently used as template for the
       synthesis of its complementary minus-strand. The proposed transient free
       plus-strand strong-stop DNA may also mediate the second template switch.
       A revised model for HIV reverse transcription will be presented.
 DE    Base Composition/GENETICS  Cell Line  DNA, Viral/*GENETICS  Human  HIV
       Infections/*MICROBIOLOGY  Models, Genetic  Repetitive Sequences, Nucleic
       Acid/GENETICS  Reverse Transcriptase/*GENETICS  Virus
       Integration/*GENETICS  Virus Replication/*GENETICS  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).