Document 0630
 DOCN  M94A0630
 TI    Activation of the NF-kappa-B p105 promoter by HTLV-I Tax.
 DT    9412
 AU    Lambert P; Ludford M; Deacon N; Doherty R; Macfarlane Burnet Centre for
       Medical Research, Fairfield,; Victoria.
 SO    Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:69 (abstract no. FB5).
       Unique Identifier : AIDSLINE ASHM5/94349025
 AB    The transactivator protein of HTLV-I, Tax, activates transcription not
       only from the HTLV-I LTR, but also from several other viral and cellular
       promoters. One of its targets is the LTR of HIV; HIV replication is
       enhanced in HTLV-I-infected cell lines, and there is increasing evidence
       that patients infected with both viruses show a more rapid progression
       to the diseased states associated with HIV infection. Activation of the
       HIV LTR by Tax is mediated by proteins of the NF-kappa B(NF-kappa
       B)/c-Rel system; there are two NF-kappa B target sites in the HIV LTR.
       Tax induces nuclear localization of cytoplasmic NF-kappa B/c-Rel,
       allowing it to bind to its target sites and activate transcription.
       There is also the possibility that Tax can increase the de novo
       synthesis of the NF-kappa B/c-Rel proteins. This seems especially likely
       given that at least some of them regulate their own synthesis. The gene
       encoding the precursor of NF-kappa B p50, p105, is one such gene.
       Reports vary on whether or not Tax can stimulate its promoter, and such
       activity may be cell type-dependent. Our preliminary results with Jurkat
       T cells indicated that a cotransfected Tax-expressing construct could
       activate a p105 promoter-CAT reporter plasmid, and we undertook further
       experiments to determine the mechanism for this.
 DE    Cell Line  Gene Amplification/*GENETICS  Gene Expression Regulation,
       Viral/PHYSIOLOGY  Gene Products, tax/*GENETICS  Human  HIV-1/GENETICS
       HTLV-I/*GENETICS  NF-kappa B/*GENETICS  Promoter Regions
       (Genetics)/*GENETICS  Protein Precursors/*GENETICS  Virus
       Replication/*GENETICS  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).