Document 0631
 DOCN  M94A0631
 TI    Mutually dependent, concurrent RNA and DNA synthesis during early phase
       HIV replication.
 DT    9412
 AU    Kok TW; Li P; Burrell CJ; Div. of Medical Virology, NCHVR, Institute of
       Medical and; Veterinary Science, Adelaide, South Australia.
 SO    Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:68 (abstract no. FB2).
       Unique Identifier : AIDSLINE ASHM5/94349024
 AB    In a cell-to-cell HIV infection model it was found that there were two
       distinct phases of induced HIV RNA synthesis. The first phase (4h - 12h
       pi) was marked by a significant increase in only the genomic-length
       viral RNA. The second, phase (24h onwards) comprised a dramatic increase
       in the levels of all three species of viral RNA. The first phase, but
       not the second phase of viral RNA induction, was abolished when
       unintegrated viral DNA synthesis was inhibited by AZT. When virus donor
       H3B cells were pre-treated with actinomycin D and mixed with untreated
       recipient Hut78 cells; the second phase, but not the first phase, of
       induced viral RNA transcription was abolished. Reverse transcription was
       not affected by actinomycin D. Full length linear unintegrated viral DNA
       was produced but viral integration was inhibited when donor and
       recipient cells were arrested in the G1 phase of the cell cycle by
       aphidicolin. Under these conditions, it was found that the second phase,
       but not the first phase of induced viral RNA synthesis was abolished.
       Taken together, these results suggest a mutually dependent concurrent
       viral RNA/DNA synthesis early after cell-to-cell transmission of HIV
       infection. The template for the first phase of viral RNA induction is
       likely to be the linear unintegrated viral DNA and the second phase may
       use newly integrated as well as proviral DNA in the donor cells as a
       more effective transcription template.
 DE    DNA, Viral/*BIOSYNTHESIS/GENETICS  Gene Expression Regulation,
       Viral/PHYSIOLOGY  Human  HIV/*GENETICS  HIV Infections/*MICROBIOLOGY
       RNA, Viral/*BIOSYNTHESIS/GENETICS  Transcription, Genetic/GENETICS
       Virus Integration/*GENETICS  Virus Replication/*GENETICS  MEETING
       ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).