Document 0634
 DOCN  M94A0634
 TI    A cis-acting repressive sequence that overlaps the Rev-responsive
       element of HIV-1 regulates nuclear retention of env mRNAs independently
       of splice signals.
 DT    9412
 AU    Brighty DW; Rosenberg M; SmithKline Beecham Pharmaceuticals, King of
       Prussia, Pennsylvania; 19406-0939.
 SO    Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:67 (abstract no. FB1).
       Unique Identifier : AIDSLINE ASHM5/94349021
 AB    The Rev protein of HIV-1 binds to an RNA structure, the Rev-Responsive
       Element (RRE), and enhances expression of the viral structural genes by
       relieving the nuclear sequestration of incompletely spliced viral
       transcripts. It has been due to the activity of cis-acting repressive
       sequence (CRS) elements and to inefficient splicing signals. We have
       demonstrated that expression of the HIV-1 envelope gene in transfected
       Drosophila cells is also completely dependent upon co-expression of Rev
       and hence, the mechanism of nuclear retention and Rev regulation is
       highly conserved in higher cell systems. We use the Drosophila system to
       identify a major CRS element which overlaps the RRE and is responsible
       for the nuclear entrapment and Rev-dependent expression of HIV-1
       envelope encoding mRNAs. Moreover, the splice signals spanning env are
       not required for nuclear retention or Rev-dependent trans-activation of
       env mRNAs. Thus the RRE and its associated complex RNA structure appear
       necessary and sufficient for both the repressive and known
       trans-activation effects of Rev regulation.
 DE    Animal  Cell Nucleus/PHYSIOLOGY  Cells, Cultured  Drosophila/GENETICS
       Gene Products, env/*GENETICS  Gene Products, rev/*GENETICS  Genetic
       Complementation Test  Human  HIV-1/*GENETICS  Repressor
       Proteins/*GENETICS  RNA Splicing/*GENETICS  Signal
       Transduction/*GENETICS  Transfection/GENETICS  MEETING ABSTRACT

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