Document 0635
 DOCN  M94A0635
 TI    Twin studies suggest a host cell genetic effect on HIV replication in
       human monocytes/macrophages.
 DT    9412
 AU    Chang J; Li S; Sullivan J; Kazazi F; Naif H; Cunningham T; Virology
       Department, Westmead Hospital, National Centre in HIV; Research, Sydney,
       Australia.
 SO    Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:66 (abstract no.
       TB10). Unique Identifier : AIDSLINE ASHM5/94349020
 AB    Biological and genetic variability is a prominent feature of HIV
       strains, especially in tropism, syncytial formation and replicative
       capacity. Preliminary studies also suggested there were variable host
       cell effects on HIV replication in monocytes. Therefore we used
       monocytes from identical (ID) twins and age matched unrelated donors
       (URD) of the same sex to study possible host genetic effects on HIV
       replication in monocytes. In all of the 7 pairs of identical twins the
       kinetics of replication (measured by extracellular HIV antigen) of 4
       different blood derived HIV strains in monocytes were insignificantly
       different within pairs. However marked and significant differences in
       HIV kinetics occurred within 5 of the 7 unrelated donor pairs. Very
       similar results were observed with monocytes from serial bleeds of the
       same donor. When studied by in situ hybridization the proportion of HIV
       infected monocytes also differed within two URD pairs. Six different
       strains of low passage monocytotropic blood isolate of HIV were
       inoculated into panels of monocytes drawn from 15 different donors and
       peak extracellular HIV titres compared. HIV strains showed patterns of
       either moderate or low level replication in most donor monocytes.
       However there was still marked variation in peak titres in a minority of
       donors. Hence differences in HIV replication kinetics between URD
       monocyte pairs contrasted with congruent patterns in ID monocytes.
       Although some strains of HIV grew well in monocytes of most donors there
       was still marked variation in some, strongly suggesting a host cell or
       genetic influence. So far no consistent genetic linkage of HIV
       replication pattern with HLA types has been observed.
 DE    Diseases in Twins/*GENETICS  Human  HIV
       Infections/*GENETICS/MICROBIOLOGY  HLA Antigens/GENETICS  In Situ
       Hybridization  Macrophages/*MICROBIOLOGY  Monocytes/*MICROBIOLOGY
       Virulence/GENETICS  Virus Replication/*GENETICS  MEETING ABSTRACT

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