Document 0636
 DOCN  M94A0636
 TI    HIV-1 integration in peripheral blood monocytes and cultured
       macrophages.
 DT    9412
 AU    Sonza S; Maerz A; Mills J; Crowe S; AIDS Pathogenesis Research Unit,
       NCHVR, Macfarlane Burnet Centre; for Medical Research, Fairfield,
       Victoria.
 SO    Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:66 (abstract no. TB9).
       Unique Identifier : AIDSLINE ASHM5/94349019
 AB    Although integration has been clearly demonstrated in HIV-1 infected
       lymphocytes, there has been only a single unconvincing report of this in
       primary macrophages. We have used various polymerase chain
       reaction-based techniques to determine whether and when this step in the
       infection process takes place in monocytes and macrophages. A novel
       procedure, Alu-PCR, which amplifies DNA between HIV-1 and ubiquitous Alu
       repeat sequences, indicated that while integrated DNA could not be
       detected even after 7 days following infection of freshly isolated
       monocytes with HIV-1Ba-L, it was detectable within 24 h of infection of
       cells which had been cultured for as little as one day. Greatest levels
       were detected at the peak of infection. This finding correlates with
       other work in our laboratory that monocytes are usually refractory to in
       vitro infection (measured by both p24 antigen production and initiation
       of reverse transcription) on the day on which they are isolated but
       become increasingly more susceptible to infection with time in culture.
       This phenomenon is not related to the level of CD4 on the cells but
       possibly to virus entry.
 DE    Human  HIV-1/*GENETICS/PATHOGENICITY  In Vitro
       Macrophages/*MICROBIOLOGY  Monocytes/*MICROBIOLOGY  Polymerase Chain
       Reaction  Virulence/GENETICS  Virus Integration/*GENETICS  Virus
       Replication/*GENETICS  MEETING ABSTRACT

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