Document 0638
 DOCN  M94A0638
 TI    Factors involved in susceptibility of macrophages but not fresh
       monocytes to HIV infection.
 DT    9412
 AU    Maerz A; Sonza S; Crowe S; Macfarlane Burnet Centre for Medical
       Research, Fairfield,; Victoria.
 SO    Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:65 (abstract no. TB8).
       Unique Identifier : AIDSLINE ASHM5/94349017
 AB    OBJECTIVES: To determine the time post isolation at which
       monocyte/macrophages show increased susceptibility to infection with
       HIV, and identify the stage of viral replication which is blocked in
       cells which are less susceptible. METHODS: Monocytes were isolated from
       HIV seronegative buffy coats by density gradient centrifugation and
       plastic adherence. Cells were infected with HIV-1 (BaL) on Day 0, 1, 2
       and 7 post isolation. HIV infection was quantitated by PCR using primer
       pairs which detect all initiated, partially completed and almost full
       length cDNA transcripts, intracellular p24 quantification by MAb
       staining and flow cytometric analysis or p24 antigen in supernatant by
       EIA. RESULTS: Freshly isolated monocytes are generally not susceptible
       to infection with HIV. At least 24 hours in culture is required before
       any sign of viral replication can be detected. CONCLUSION: There is a
       block to HIV infection in freshly isolated monocytes which is overcome
       when cells have been cultured for 24 hours. Further studies are ongoing
       to compare internalization of virus, linearization of DNA, translocation
       of DNA from cytoplasm to nucleus and NFkB activity in the two
       populations.
 DE    Cells, Cultured  Human  HIV/*PATHOGENICITY  HIV Seronegativity
       Macrophages/*MICROBIOLOGY  Monocytes/*MICROBIOLOGY  Virulence  Virus
       Replication/*PHYSIOLOGY  MEETING ABSTRACT

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