Document 0639
 DOCN  M94A0639
 TI    Uptake and expression of HIV-1 B isolated thymic dendritic cells.
 DT    9412
 AU    Lowe M; Sotzic F; Shortman K; Cameron P; Macfarlane Burnet Centre for
       Medical Research, Fairfield,; Victoria.
 SO    Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:64 (abstract no. TB6).
       Unique Identifier : AIDSLINE ASHM5/94349016
 AB    Dendritic cells (DC) are the major antigen presenting cells for the
       induction of T cell mediated immune responses. We have found DC isolated
       from peripheral blood by sorting are CD4- and do not allow viral entry
       by T cell or macrophage tropic isolates of HIV-1. DC are functionally
       and phenotypically heterogeneous and immature blood DC and DC from other
       sites express CD4. PCR was used to detect entry of macrophage and T cell
       tropic HIV-1 into DC expressing CD4. Thymic DC are CD4+ dendritic cells
       that develop with thymocytes from a common precursor. Thymic DC were
       purified to near homogeneity and pulsed with different HIV-1 isolates.
       After 24-48 hrs provirus was detected for macrophage tropic Ba-L and
       YU-2 virus strains, but not T cell tropic isolates 228200 or IIIB. In
       addition to the previously described ability of mature DC to carry virus
       and infect T cells during antigen specific responses, infected CD4+ DCs
       may be a continuing source of virus or provide signals for induction of
       energy or apoptosis of responding T cells.
 DE    Dendritic Cells/IMMUNOLOGY/*MICROBIOLOGY  Human
       HIV-1/CLASSIFICATION/*IMMUNOLOGY  Lymphocyte Transformation/IMMUNOLOGY
       T-Lymphocytes/IMMUNOLOGY/*MICROBIOLOGY  Thymus
       Gland/IMMUNOLOGY/*MICROBIOLOGY  T4 Lymphocytes/IMMUNOLOGY/MICROBIOLOGY
       MEETING ABSTRACT

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