Document 0643
 DOCN  M94A0643
 TI    Isolation and zidovudine sensitivity of HIV-1 isolated from post-mortem
       tissue.
 DT    9412
 AU    McGavin CH; Land SA; Birch CJ; Virology Department, Fairfield Hospital,
       Victoria.
 SO    Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:62 (abstract no. TB1).
       Unique Identifier : AIDSLINE ASHM5/94349012
 AB    We attempted to isolate HIV from post-mortem tissues (CSF, spleen,
       spinal cord, brain and lymph node) of 64 patients. The overall isolation
       rate was low (48%) compared to a rate of 95% from peripheral blood
       mononuclear leucocytes (PBMC's) from the same patients when they were
       alive. This difference appeared to be due to several factors including
       delay post death in collecting the tissues, the tissue site and the
       pathologist who performed the post-mortem. Usually the AZT
       susceptibility of the isolate from the postmortem material was the same
       as the PBMC isolate. However, in some patients whose PBMC isolate had
       reverted to AZT sensitivity after cessation of therapy, virus in some
       tissues remained resistant. In addition, patients whose PBMC isolate
       first became resistant to AZT at a time close to their death still had
       sensitive virus in some tissues. These results will be discussed in the
       light of our current understanding of the pathogenesis of HIV.
 DE    Brain/MICROBIOLOGY/PATHOLOGY  Cerebrospinal Fluid/MICROBIOLOGY  Drug
       Resistance, Microbial  Human  HIV Infections/*PATHOLOGY  HIV-1/*DRUG
       EFFECTS/ISOLATION & PURIF  Lymph Nodes/MICROBIOLOGY/PATHOLOGY
       Monocytes/MICROBIOLOGY/PATHOLOGY  Spleen/MICROBIOLOGY/PATHOLOGY  *Virus
       Cultivation  Zidovudine/*PHARMACOLOGY  MEETING ABSTRACT

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