Document 0703
 DOCN  M94A0703
 TI    IgM antibodies against complex glycolipids of peripheral myelin in human
       immunodeficiency virus (HIV) infection.
 DT    9412
 AU    Petratos S; Papadopoulos R; Turnbull V; Ayers M; Gonzales MF; Department
       of Anatomical Pathology, Royal Melbourne Hospital,; Parkville, Victoria.
 SO    Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:30 (abstract no. TC9).
       Unique Identifier : AIDSLINE ASHM5/94348952
 AB    IgM antibodies against complex glycolipids, in particular GM1, GD1b and
       GQ1b gangliosides have been detected in the plasma of patients with
       acute and chronic inflammatory demyelinating polyneuropathy, multi-focal
       motor neuropathy and motor neurone disease. Inflammatory demyelinating
       neuropathy is one of several neuropathies that occur in patients with
       HIV infection. To determine the frequency of IgM antibodies against
       peripheral myelin glycolipids in HIV infection, 118 plasma samples were
       screened by enzyme-linked immunoassay (ELISA) and further analysed by
       Western blotting, indirect immunofluorescence and thin layer
       chromatography overlay. Thirty seven percent of samples showed IgM
       reactivity with purified myelin by ELISA, compared with only an
       occasional sample among 200 healthy blood donors. Reactivity could be
       abolished by prior incubation with purified myelin. No reactivity with
       myelin proteins was detected in any of the ELISA-positive samples.
       Reactivity with galactocerebroside was detected in 16 of 21
       ELISA-positive samples and a further 7 samples showed reactivity with
       GD1b and GM1 gangliosides. These data suggest that immune damage to
       peripheral myelin in HIV infection may be mediated, in part, by IgM
       antibodies against glycolipids.
 DE    Autoantibodies/*ANALYSIS  Demyelinating Diseases/DIAGNOSIS/IMMUNOLOGY
       Enzyme-Linked Immunosorbent Assay  G(M1) Ganglioside/*IMMUNOLOGY
       Gangliosides/*IMMUNOLOGY  Human  HIV Infections/*IMMUNOLOGY
       IgM/*ANALYSIS  Myelin Proteins/*IMMUNOLOGY  Peripheral Nerves/IMMUNOLOGY
       MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).