Document 0728
 DOCN  M94A0728
 TI    The study of Nef mutant clones of human immunodeficiency virus (HIV).
 DT    9412
 AU    Arunagiri CK; Peden K; Azad A; McPhee D; Biomolecular Research
       Institute, Parkville.
 SO    Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:108 (poster no. 67).
       Unique Identifier : AIDSLINE ASHM5/94348927
 AB    A study was initiated to determine the function of Nef auxiliary protein
       replication of HIV. A molecular clone of HIV, LAI, was selected and two
       truncated (termination at amino acid 46 and 88) nef mutant clones
       constructed. The proviral DNA of the mutants and wild type were used to
       transfect Hela cells. Virus produced in Hela cells was passaged onto
       primary T4 lymphocytes and virus stocks generated for replication
       kinetic studies. The cells were infected at different multiplicities of
       infection (MOI). The results indicate that the nef mutant clones are
       slower in their ability to replicate at low MOI in both primary cells.
       However, at MOI of 1, there was no difference in the replication
       kinetics of wild type and nef mutants. This highlights the different
       effects of the Nef if different MOI are used. We conclude that the Nef
       protein is a positive factor at low MOI.
 DE    *Cloning, Molecular  Genes, nef/*GENETICS  Hela Cells  Human
       HIV/*GENETICS  Mutation/*GENETICS  Transfection/GENETICS  T4
       Lymphocytes/MICROBIOLOGY  Virus Replication/GENETICS  MEETING ABSTRACT

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