Document 0729
 DOCN  M94A0729
 TI    HIV regulatory and accessory gene mRNAs.
 DT    9412
 AU    Smith J; Penschow J; Deacon NJ; AIDS Molecular Biology Lab, Macfarlane
       Burnet Centre for Medical; Research, Fairfield, Vic.
 SO    Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:108 (poster no. 66).
       Unique Identifier : AIDSLINE ASHM5/94348926
 AB    The regulatory and accessory proteins of human immunodeficiency virus
       type 1 (HIV-1) are produced from singly or multiply spliced mRNAs. We
       have used HIV-1 specific oligonucleotide primer pairs in a polymerase
       chain reaction procedure on cDNA reverse transcribed from cytoplasmic
       RNA extracted from lymphocytic cell lines infected with HIV-1 228200.
       The amplified products were analysed by hybridisation with spliced
       junction-specific oligonucleotide probes to determine splice
       donor/splice acceptor combination utilisation and the temporal
       expression of different mRNA species. This work has led to the
       identification of two novel splice acceptor sites which are used in the
       early production of a series of distinct RNAs upon acute infection.
       These RNAs have also been demonstrated in infected cells by in-situ
       hybridisation with the splice junction specific probes. Work is
       currently being carried out to determine the function of these RNAs.
 DE    Cell Line, Transformed  Genes, Regulator/*GENETICS  Human
       HIV-1/*GENETICS  In Situ Hybridization  RNA Probes  RNA
       Splicing/*GENETICS  RNA, Messenger/*GENETICS  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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