Document 0730
 DOCN  M94A0730
 TI    Detection of HIV-1(HTLV-I) pseudotypes.
 DT    9412
 AU    Lawson V; Doultree J; Lee JY; Marshall J; McPhee D; Macfarlane Burnet
       Centre for Medical Research, NCHVR, Fairfield,; Vic.
 SO    Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:107 (poster no. 65).
       Unique Identifier : AIDSLINE ASHM5/94348925
 AB    The mixing of envelope glycoproteins in cells infected with two viruses
       may result in the formation of pseudotypes. Several studies have
       indicated that human immunodeficiency virus (HIV) may form pseudotypes
       with several viral species including human T-cell leukemia virus
       (HTLV-I), murine amphotropic virus and HIV-2. The resulting viral
       species have been shown to have an expanded host range which reflects
       the tropism of the acquired glycoproteins and results in more aggressive
       disease progression. In the majority of these studies pseudotypes have
       been artificially developed and the evidence for their formation based
       almost entirely on indirect immunological evidence. We present here a
       novel immunoprecipitation technique for the detection of HIV-1(HTLV-I)
       pseudotypes arising from HIV-1 infection of the HTLV-I transformed MT-2
       cell line, as well as a technique for direct detection of pseudotypes
       which involves immune labelling of viral envelope glycoproteins in
       association with thin section electron microscopy. The HTLV-I gp46
       surface glycoprotein was found in association with HIV-I core proteins
       in mature virus particles thus confirming previous findings and directly
       demonstrating pseudotype particles.
 DE    Cell Line, Transformed  Human  HIV Infections/MICROBIOLOGY
       HIV-1/CLASSIFICATION/*PHYSIOLOGY/PATHOGENICITY
       HTLV-I/CLASSIFICATION/*PHYSIOLOGY/PATHOGENICITY  Precipitin Tests
       Virulence/PHYSIOLOGY  MEETING ABSTRACT

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