Document 0732
 DOCN  M94A0732
 TI    HIV strains and the proliferative response of mononuclear cells from
       normal donors.
 DT    9412
 AU    Adams S; Todd B; Pope J; NCHVR, Royal Children's Hospital, Brisbane,
       Qld.
 SO    Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:106 (poster no. 62).
       Unique Identifier : AIDSLINE ASHM5/94348923
 AB    Recent interest has focused on the different biological properties of
       HIV-1 isolates. Non-syncytium-inducing (NSI) isolates, detectable at all
       stages of HIV infection, are preferentially monocytotropic in contrast
       to SI isolates which precede symptoms of AIDS and are T-cell tropic. We
       are examining the effects of NSI and SI virus strains on the
       proliferative responses of seronegative PBMC in vitro. Peripheral
       lymphocytes from seronegative donors were infected with NSI and SI virus
       at similar MOI, and PHA, autologous EBV LCL and tetanus toxin were added
       at 0, 3, 7 and 14 days postinfection (PI). At 3, 7 or 14 days
       responsiveness to PHA was reduced by as much as 56-66% in the cells of
       donor WC infected with the SI isolate compared to the response of cells
       infected with the NSI isolate. The responses to EBV antigen were also
       lower in cells infected with the SI isolate at 3, 7 or 14 days PI. This
       observation is being further studied to determine conclusively whether
       SI virus interferes with proliferative responsiveness in vitro.
 DE    Blood Donors  Cytopathogenic Effect, Viral/IMMUNOLOGY  Human  HIV
       Infections/*IMMUNOLOGY  HIV Seronegativity/IMMUNOLOGY
       HIV-1/CLASSIFICATION/*IMMUNOLOGY/PATHOGENICITY  In Vitro  Lymphocyte
       Transformation/*IMMUNOLOGY  T-Lymphocytes/*IMMUNOLOGY  Virulence
       MEETING ABSTRACT

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