Document 0733
 DOCN  M94A0733
 TI    CD4-bearing human thymic dendritic cells as potential targets for HIV
       infection.
 DT    9412
 AU    Sotzik F; Shortman K; Walter and Eliza Hall Institute of Medical
       Research, Royal; Melbourne Hospital, Parkville, Victoria.
 SO    Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:106 (poster no. 63).
       Unique Identifier : AIDSLINE ASHM5/94348922
 AB    The intention of the project was the isolation of CD4-bearing
       T-precursor thymocytes. This laboratory has found these to be the
       earliest T-precursors in the murine thymus with T cell receptor genes in
       the germline, unrearranged state. In order to identify the human
       equivalent, CD34 and CD7 expression has been examined on cells lacking
       certain markers of mature T cells. CD7+34+thymocytes bearing differing
       levels of CD2 were only observed amongst CD4-cells. No CD4+ cell
       resembling the earliest murine T-precursor was found. However a
       CD4(high) human thymic dendritic cell was discovered, which was class II
       MHC(high) class I MHC(high) CD44+ CD1-2-3-7-8-16-34-, negative for
       monocyte, NK and B cell surface markers. More peripheral blood cells
       (PBL) (i.e. 10(2)x) than dendritic cells were required to stimulate T
       cells equally well in a mixed lymphocyte reaction (MLR) culture system.
       Cells sorted into simple culture medium in Terasaki wells and incubated
       at 37 degrees C assumed dendritic morphology after 2 to 3 hours. In
       collaboration with the NCHVR and Macfarlane Burnet Centre, the capacity
       of these cells to be infected with HIV will be assessed.
 DE    Antigens, CD4/*ANALYSIS  Cells, Cultured  Dendritic
       Cells/IMMUNOLOGY/*MICROBIOLOGY  Human  HIV/*IMMUNOLOGY/PATHOGENICITY
       Thymus Gland/*CYTOLOGY  T4 Lymphocytes/IMMUNOLOGY/*MICROBIOLOGY
       Virulence  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).