Document 0734
 DOCN  M94A0734
 TI    Transcription factors in HIV-1 infection.
 DT    9412
 AU    Kedar P; Arden KE; Todd BJ; Pope JH; NCHVR, Royal Children's Hospital,
       Brisbane, Qld.
 SO    Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:105 (poster no. 61).
       Unique Identifier : AIDSLINE ASHM5/94348921
 AB    Transcriptional regulation involves the interaction of enhancer-promoter
       regions with cellular binding proteins. NF-kB is a nuclear
       transcriptional factor which binds and mediates transcription from the
       sequences in the promoter region. In our system we have studied NF-kB
       and related proteins in PHA stimulated cord blood mononuclear cells. In
       this model the cells were treated with rIL2 and with or without
       DEAE-dextran, and nuclear extracts were made. Binding of NF-kB enhancer
       region sequences with these extracts were studied by gel shift assays.
       As well as NF-kB, another protein NF-TIM was detected in large amounts.
       This NF-TIM also binds to NF-kB region even when it is mutated (GGG to
       CTC) but binding is decreased with double mutated enhancer (GGG to CTC
       and CCG to AAC). Further studies of the role of NF-TIM in HIV-1
       replication are in progress with UV crosslinking, footprinting, and
       cloning of enhancer region and its mutants in a reporter gene system.
 DE    Cell Line  Human  HIV Enhancer/GENETICS  HIV Infections/*MICROBIOLOGY
       HIV-1/*GENETICS  Monocytes/MICROBIOLOGY  Mutation/GENETICS  NF-kappa
       B/GENETICS  Transcription Factors/*GENETICS  Virus Replication/*GENETICS
       MEETING ABSTRACT

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       protected by Copyright Law (Title 17, U.S.Code).