Document 0735
 DOCN  M94A0735
 TI    Primary T-cell response to HIV-1 in vitro.
 DT    9412
 AU    Todd B; Pope J; NCHVR, Royal Children's Hospital, Brisbane, Qld.
 SO    Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:105 (poster no. 60).
       Unique Identifier : AIDSLINE ASHM5/94348920
 AB    HIV-I-specific, MHC-restricted cytotoxic T cells may be detected
       directly in peripheral blood (PB) of HIV-1-infected patients, and
       cytotoxic T-cell lines can be established in vitro. CD8+ T cells
       predominate, and epitopes exist in most viral antigens. Generation of
       cytotoxic T cells from the PB mononuclear cells from uninfected donors
       has received less attention. Our aim is to characterise the primary
       cellular immune response to HIV-1 infection in vitro. The approach is to
       generate T-cell colonies and amplify T-cell lines and clones. These
       T-cells are phenotyped (cell markers) and their HIV-1 specificity
       determined by use of autologous targets infected with HIV-1 or with
       vaccinia constructs expressing HIV-1 antigens. So far, 92 T-cell lines
       and 20 clones have been established in response to stimulation with live
       or inactivated HIV-1. Both CD4+ and CD8+ T cells have been generated.
       Cytotoxicity tests have so far yielded 6 T-cell lines with putative
       HIV-1 specificity for detailed study.
 DE    Cell Line  Cytotoxicity Tests, Immunologic  Human  HIV-1/*IMMUNOLOGY
       Immunophenotyping  In Vitro  T-Lymphocytes/*IMMUNOLOGY  T-Lymphocytes,
       Cytotoxic/*IMMUNOLOGY  MEETING ABSTRACT

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