Document 0737
 DOCN  M94A0737
 TI    Cytokine dysregulation associated with the course of HIV infection.
 DT    9412
 AU    Fan J; Bass H; Nguyen T; Fahey JL; National Centre for HIV Virology
       Research, Institute of Medical; and Veterinary Science, Adelaide, South
       Australia.
 SO    Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:104 (poster no. 58).
       Unique Identifier : AIDSLINE ASHM5/94348918
 AB    By using quantitative RT-PCR, elevated levels of IFN gamma mRNA and
       lowered IL-2 mRNA were found in PBMC of HIV-seropositive men with CD4 T
       cells over 500/mm3 (mean:647/mm3) while IL-4 and IL-10 mRNAs did not
       change significantly. Men whose CD4 levels fell more than 200/mm3 in the
       two following years showed further changes in lymphokine mRNA levels.
       Furthermore, changes in cytokine gene expression were markedly different
       in CD4 and CD8 T cells from HIV-seropositive men. On a per cell basis,
       mRNA levels of IFN gamma, IL-10 and TNF alpha increased approximately
       three times in CD4 T cells isolated from HIV seropositive subjects (p <
       0.05) while IL-2 gene expression showed no significant change. Cytokine
       production in CD8 T cells, however, changed little following HIV
       infection. After stimulation of PBMC with PHA, PWM, and rIL-2; IL-2 and
       IL-2R were found lower, but IL-10 and TNF alpha found higher in
       seropositive subjects as compared to seronegative individuals. Cytokines
       play a central role in the performance and regulation of the human
       immune system. The altered balance of cytokine production is likely to
       contribute to the immune dysfunction in HIV-infection.
 DE    Cytokines/*GENETICS  CD4-CD8 Ratio  Gene Expression Regulation,
       Viral/PHYSIOLOGY  Human  HIV Infections/GENETICS/*IMMUNOLOGY  HIV
       Seronegativity/GENETICS/IMMUNOLOGY  Interferon Type II/GENETICS
       Interleukin-10/GENETICS  Interleukin-2/GENETICS  Interleukin-4/GENETICS
       Leukocyte Count  RNA, Messenger/GENETICS  T4 Lymphocytes/IMMUNOLOGY
       MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).