Document 0738
 DOCN  M94A0738
 TI    Role the immunodeficiency virus nef gene and the production in CNS
       tropism.
 DT    9412
 AU    Smith K; Deacon NJ; McPhee DA; Brew B; AIDS Molecular Biology Unit,
       Macfarlane Burnet Centre, Vic.,; Australia.
 SO    Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:103 (poster no. 56).
       Unique Identifier : AIDSLINE ASHM5/94348917
 AB    Particular strains of HIV may dominate the CNS of infected individuals
       and it is possible that the HIV Nef protein plays a role in
       differentiating CNS and peripheral blood isolates. To address this, 6
       pairs of coincident isolates from the CSF and peripheral blood of
       patients with AIDS or ARC were analysed for their ability to replicate
       in primary blood mononuclear cells and primary macrophages. Peripheral
       blood isolates appeared to have enhanced replication in all the cell
       types examined but the replicative abilities of paired isolates were
       very similar. CSF isolates were less likely to induce CPE and syncytia
       in PBMC and macrophage cultures. Isolates were examined for their
       ability to express the Nef protein by flow cytometry and immunoblotting
       and the nef gene of isolates was PCR amplified and sequenced. Most
       isolates appeared to express Nef and sequence analysis revealed little
       difference in nef between CSF and blood isolates. No differences between
       Nef of CSF and peripheral blood isolates were apparent by parameters
       used in this study.
 DE    Acquired Immunodeficiency Syndrome/MICROBIOLOGY  AIDS Dementia
       Complex/*MICROBIOLOGY  AIDS-Related Complex/MICROBIOLOGY
       Brain/MICROBIOLOGY  Cerebrospinal Fluid/MICROBIOLOGY  Cytopathogenic
       Effect, Viral/GENETICS  Flow Cytometry  Genes, nef/*GENETICS  Human
       HIV/*GENETICS  Immunoblotting  Polymerase Chain Reaction  Sequence
       Analysis  Virus Replication/GENETICS  MEETING ABSTRACT

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