Document 0739
 DOCN  M94A0739
 TI    Characterisation of HIV-1 LTR DNA-binding proteins.
 DT    9412
 AU    Peters A; Deacon NJ; AIDS Molecular Biology Laboratory, Macfarlane
       Burnet Centre for; Medical Research, Fairfield, Vic., Australia.
 SO    Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:103 (poster no. 57).
       Unique Identifier : AIDSLINE ASHM5/94348916
 AB    An investigation into the characterisation of mammalian nuclear proteins
       which bind to the HIV-1 LTR U3 region has been carried out using a
       series of overlapping 40-mer oligonucleotides. These oligonucleotides
       were synthesised to correspond to the HXB2 sequence from n.t. -210 to
       n.t. -454. Two oligonucleotides have shown a number of specific binding
       interactions in mobility shift assays, U.V. cross-linked mobility shift
       assays and south-western analysis. Oligonucleotide 7 (n.t. -210 to n.t.
       -250) has at least 4 specific interactions and oligonucleotide 13(n.t.
       -410 to n.t. 454) has at least 2 specific interactions. The region of
       oligonucleotide 7 is thought to bind ILF (Li et al, 1991) and possibly
       members of the ets family. No protein binding has been reported in the
       region of oligonucleotide 13. Reference: Li P, et al. 1991 P.N.A.S. USA
       88; 7739-7743.
 DE    DNA-Binding Proteins/*GENETICS  Human  HIV-1/*GENETICS
       Oligonucleotides/GENETICS  Repetitive Sequences, Nucleic Acid/*GENETICS
       MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).