Document 0741
 DOCN  M94A0741
 TI    Characterisation of putative steroid response elements in the long
       terminal repeat of HIV-1.
 DT    9412
 AU    Barnes N; Deacon N; Doherty R; Macfarlane Burnet Centre, Fairfield, Vic.
 SO    Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:102 (poster no. 55).
       Unique Identifier : AIDSLINE ASHM5/94348914
 AB    Motifs with sequence homology to steroid hormone response elements have
       been identified in the upstream half of the LTR of HIV by several
       investigators, and nuclear factors have been identified in HeLa cell
       extracts which bind to these regions. The motifs appear conserved in HIV
       consensus sequences, and to a variable degree in strains of SIV, CAEV,
       EIAV and HTLV-I. We have studied the functional capacity of the HIV
       motifs by transfecting MCF-7 cells with the HIV LTR reporter gene
       construct pBENNCAT (Cells were cotransfected with pSVTat72). The
       estrogen responsive construct pVITtkCAT was used as control. The effect
       of estrogen on HIV LTR controlled transcription was assessed by the CAT
       activity present in cell lysates. Modest increases in CAT activity were
       observed in estrogen treated cells. In addition, a nuclear protein
       present in extracts of estrogen treated MCF-7 and HeLa ER cells was
       shown to bind to a synthetic oligonucleotide probe containing the
       ERE-like HIV sequence. Further characterisation of this protein is
       underway. Mutations have been introduced into the ERE-like region to
       abolish the element and to substitute the respective RE consensus
       sequence for the wild type HIV sequence in the reporter gene constructs.
 DE    Estrogens/*PHARMACOLOGY  Hela Cells  Human  HIV-1/*DRUG EFFECTS/GENETICS
       Receptors, Estrogen/*DRUG EFFECTS/GENETICS  Repetitive Sequences,
       Nucleic Acid/*DRUG EFFECTS/GENETICS  Sequence Homology  Transcription,
       Genetic/DRUG EFFECTS  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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