Document 0743
 DOCN  M94A0743
 TI    Lymphocyte subset analysis by Boolean algebra: a phenotypic approach
       using a cocktail of 5 antibodies and 3 colour immunofluorescence.
 DT    9412
 AU    Hunter S; Peters L; Wotherspoon J; Crowe S; Flow Cytometry Unit,
       Macfarlane Burnet Centre for Medical; Research, Fairfield Hospital,
       Victoria, Australia.
 SO    Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:101 (poster no. 52).
       Unique Identifier : AIDSLINE ASHM5/94348912
 AB    A rapid method has been developed whereby total CD3+ T-cells, CD4+
       T-cells (CD3+CD4+), CD8+ T-cells (CD3+CD8+), putative gamma
       delta-receptor-T-cells (CD3+CD4-CD8-) and T-cells that are CD3+CD4+CD8+
       as well as B-lymphocytes and NK-cells can be enumerated after staining
       in a single tube. Whole blood specimens are labelled with a mixture of
       antibodies: FITC-CD4 and CD19, PE-CD8 and CD16, and either peridinin
       chlorophyll protein (PerCP) or allophycocyanin (APC) labelled CD3 for
       use on a Becton Dickinson FACScan or FACStar Plus flow cytometer
       respectively. Data were analysed with LYSYS-II software package and all
       of the lymphocyte subset values were determined by Boolean algebra. Our
       study has shown that this new procedure is statistically equivalent to a
       standard analysis procedure (SimulSET lymphocyte subset analysis), is
       less time consuming and more cost effective.
 DE    B-Lymphocyte Subsets/*IMMUNOLOGY  Flow Cytometry  *Fluorescent Antibody
       Technique  Human  Immunophenotyping/*METHODS  Leukocyte Count/*METHODS
       Software  T-Lymphocyte Subsets/*IMMUNOLOGY  MEETING ABSTRACT

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